Evidence map›Paper›PMID 39456862›Full record

ArticleInternational journal of molecular sciences2024

Quantitative Trait Locus Mapping Combined with RNA Sequencing Identified Candidate Genes for Resistance to Powdery Mildew in Bitter Gourd (

Rukui Huang, Jiazuo Liang, Xixi Ju, Yuhui Huang, Xiongjuan Huang, Xiaofeng Chen, Xinglian Liu, Chengcheng Feng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Rukui HuangVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.ORCID 0000-0002-5407-7244
Jiazuo LiangVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Xixi JuVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Yuhui HuangVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Xiongjuan HuangVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Xiaofeng ChenVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Xinglian LiuVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Chengcheng FengVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.

Funding

China Agriculture Research System CARS-23-G53Guangxi Academy of Agricultural Sciences Basic research project GAST2021YT099Guangxi Science and Technology Key Project Guike AA23023001The National Natural Science Foundation of China 32060686
6 · The paper itself

Abstract

Improving the powdery mildew resistance of bitter gourd is highly important for achieving high yield and high quality. To better understand the genetic basis of powdery mildew resistance in bitter gourd, this study analyzed 300 lines of recombinant inbred lines (RILs) formed by hybridizing the powdery mildew-resistant material MC18 and the powdery mildew-susceptible material MC402. A high-density genetic map of 1222.04 cM was constructed via incorporating 1,996,505 SNPs generated by resequencing data from 180 lines, and quantitative trait locus (QTL) positioning was performed using phenotypic data at different inoculation stages. A total of seven QTLs related to powdery mildew resistance were identified on four chromosomes, among which qPm-3-1 was detected multiple times and at multiple stages after inoculation. By selecting 18 KASP markers that were evenly distributed throughout the region, 250 lines and parents were genotyped, and the interval was narrowed to 207.22 kb, which explained 13.91% of the phenotypic variation. Through RNA-seq analysis of the parents, 11,868 differentially expressed genes (DEGs) were screened. By combining genetic analysis, gene coexpression, and sequence comparison analysis of extreme materials, two candidate genes controlling powdery mildew resistance in bitter gourd were identified (

Indexed as

Chromosome MappingDisease ResistanceMomordica charantiaPlant DiseasesQuantitative Trait LociAscomycotaChromosomes, PlantGene Expression Regulation, PlantGenes, PlantPhenotypePolymorphism, Single NucleotideSequence Analysis, RNAcandidate geneMomordica charantiapowdery mildewQTLRNA-seq

Identifiers

PMID39456862
PMCPMC11508001

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.