Evidence map›Paper›PMID 39457407›Full record

ArticleGenes2024

Selection of Reference Genes and

Ana Carvalho, Christina Crisóstomo, Fernanda Leal, José Lima-Brito

Abstract read
In one paragraph

Article in Genes, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Ana CarvalhoPlant Cytogenomics Laboratory, University of Trás-os-Montes and Alto Douro (UTAD), Laboratorial Complex, Room A1.09, Quinta de Prados, 5000-801 Vila Real, Portugal.ORCID 0000-0002-6049-9291
Christina CrisóstomoPlant Cytogenomics Laboratory, University of Trás-os-Montes and Alto Douro (UTAD), Laboratorial Complex, Room A1.09, Quinta de Prados, 5000-801 Vila Real, Portugal.ORCID 0009-0009-0744-5129
Fernanda LealDepartment of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro (UTAD), Laboratorial Complex, Quinta de Prados, 5000-801 Vila Real, Portugal.
José Lima-BritoPlant Cytogenomics Laboratory, University of Trás-os-Montes and Alto Douro (UTAD), Laboratorial Complex, Room A1.09, Quinta de Prados, 5000-801 Vila Real, Portugal.ORCID 0000-0001-6204-009X

Funding

Fundação para a Ciência e a Tecnologia DL 57/2016/CP1378/CT0003 (doi:10.54499/DL57/2016/CP1378/CT0003)Fundação para a Ciência e a Tecnologia UIDB/04033/2020 (https://doi.org/10.54499/UIDB/04033/2020)Portugal 2020 under the project INNOVINE&WINE - Vineyard and Wine Innovation Platform (NORTE-01-0145-FEDER-000038, research line Viticulture BPD/UTAD/INNOVINE&WINE/593/2016
6 · The paper itself

Abstract

background"Touriga Franca" (TF) and "Touriga Nacional" (TN) are grapevine varieties cultivated in the 'Douro Superior' subregion (Northern Portugal) that experience stressful environmental conditions during the summer.

objectivesAiming to profile the expression of stress-responsive genes by quantitative real-time PCR (qPCR) in TF and TN plants growing naturally, three candidate reference genes were first tested under controlled conditions.

methodsTo simulate a summer's day, TF and TN in vitro plants were exposed to 32 °C-3 h (heat acclimation) and 42 °C-1 h (severe heat stress, HS) followed by two recovery periods (32 °C-3 h and 24 °C-24 h). Leaf samples were collected at the end of each phase. Control plants were kept at 24 °C.

resultsAmong the candidate reference genes, the

conclusionsTN responded faster than TF to the induced heat shocks. The

Indexed as

Gene Expression Regulation, PlantHeat-Shock ResponsePlant ProteinsVitisGene Expression ProfilingHeat-Shock ProteinsPlant LeavesReal-Time Polymerase Chain ReactionReference StandardsHeat-Shock ProteinsPlant Proteinsgene expressionheat shock proteinin vitro culturequantitative real-time PCR (qPCR)Ubiquitin-conjugating enzyme (UBC) geneVacuolar ATPase subunit G (VAG) gene

Identifiers

PMID39457407
PMCPMC11507026

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.