Evidence map›Paper›PMID 39459031›Full record

ArticlePharmaceuticals (Basel, Switzerland)2024

Assessing the Neurodevelopmental Impact of Fluoxetine, Citalopram, and Paroxetine on Neural Stem Cell-Derived Neurons.

Kimia Hosseini, Andrea Cediel-Ulloa, Mohamed H Al-Sabri, Anna Forsby, Robert Fredriksson

Abstract read
In one paragraph

Article in Pharmaceuticals (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kimia HosseiniDepartment of Pharmaceutical Bioscience, Uppsala University, 751 24 Uppsala, Sweden.
Andrea Cediel-UlloaDepartment of Organismal Biology, Uppsala University, 752 36 Uppsala, Sweden.ORCID 0000-0002-0237-3594
Mohamed H Al-SabriDepartment of Pharmaceutical Bioscience, Uppsala University, 751 24 Uppsala, Sweden.ORCID 0000-0002-9542-8653
Anna ForsbyDepartment of Biochemistry and Biophysics, Stockholm University, 106 91 Stockholm, Sweden.ORCID 0000-0001-6298-201X
Robert FredrikssonDepartment of Pharmaceutical Bioscience, Uppsala University, 751 24 Uppsala, Sweden.

Funding

Åhlens Foundation (FO2022-0230)Gunvor and Josef Anérs (FB23-012)Swedish Research Council (2019-01811)
6 · The paper itself

Abstract

BACKGROUND/

objectivesMany pregnant women globally suffer from depression and are routinely prescribed selective serotonin reuptake inhibitors (SSRIs). These drugs function by blocking the re-uptake of serotonin by the serotonin transporter (SERT) into neurons, resulting in its accumulation in the presynaptic cleft. Despite a large amount of research suggesting a potential link to neurodevelopmental disorders in children whose mothers took these drugs during pregnancy, their possible adverse effects are still debated, and results are contradictory. On the other hand, there is an immediate need for improved cell-based models for developmental neurotoxicity studies (DNT) to minimize the use of animals in research.

methodsIn this study, we aimed to assess the effects of clinically relevant concentrations of paroxetine (PAR), fluoxetine (FLX), and citalopram (CIT)-on maturing neurons derived from human neural stem cells using multiple endpoints.

resultsAlthough none of the tested concentrations of FLX, CIT, or PAR significantly affected cell viability, FLX (10 µM) exhibited the highest reduction in viability compared to the other drugs. Regarding neurite outgrowth, CIT did not have a significant effect. However, FLX (10 µM) significantly reduced both mean neurite outgrowth and mean processes, PAR significantly reduced mean processes, and showed a trend of dysregulation of multiple genes associated with neuronal development at therapeutic-relevant serum concentrations.

conclusionsTranscriptomic data and uptake experiments found no SERT activity in the system, suggesting that the adverse effects of FLX and PAR are independent of SERT.

Indexed as

DNThNSCsoff-targetSSRIs

Identifiers

PMID39459031
PMCPMC11510426

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.