Evidence map›Paper›PMID 39460910›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Serum-Free and Protein-Free Media for the Cultivation of Recombinant Chinese Hamster Ovary (CHO) Cell Lines.

Berta Capella Roca, Justine Meiller, Padraig Doolan, Martin Clynes

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Berta Capella RocaNational Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland.
Justine MeillerNational Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland.
Padraig DoolanNational Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland.
Martin ClynesNational Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland. martin.clynes@dcu.ie.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We describe the preparation of two cell culture media formulations for the culture in suspension of Chinese hamster ovary (CHO) cell lines. The first medium, Cell growth SFM Medium, is a serum-free medium designed to maintain cell growth with high-viability profiles. The second corresponds to a protein-free version optimized to increase CHO recombinant protein production (Production PFM Medium). Considerations on the use of these formulations for other cell culture assays (such as transfections) are also described.

Indexed as

Cell Culture TechniquesCricetulusRecombinant ProteinsAnimalsCell ProliferationCHO CellsCricetinaeCulture MediaCulture Media, Serum-FreeCulture MediaCulture Media, Serum-FreeRecombinant ProteinsProtein-free mediaRecombinant Chinese hamster ovary cellsSerum-free media

Identifiers

PMID39460910

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.