Evidence map›Paper›PMID 39466357›Full record

ArticleInterdisciplinary sciences, computational life sciences2025

BES-Designer: A Web Tool to Design Guide RNAs for Base Editing to Simplify Library.

Qian Zhou, Qian Gao, Yujia Gao, Youhua Zhang, Yanjun Chen, Min Li, Pengcheng Wei, Zhenyu Yue

Abstract read
PubMed Publisher
In one paragraph

Article in Interdisciplinary sciences, computational life sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Qian Zhou *School of Information and Artificial Intelligence, Anhui Provincial Engineering Research Center for Beidou Precision Agriculture Information, Anhui Agricultural University, Hefei, 230036, China.ORCID http://orcid.org/0009-0003-1022-9845
Qian Gao *School of Information and Artificial Intelligence, Anhui Provincial Engineering Research Center for Beidou Precision Agriculture Information, Anhui Agricultural University, Hefei, 230036, China.
Yujia GaoSchool of Information and Artificial Intelligence, Anhui Provincial Engineering Research Center for Beidou Precision Agriculture Information, Anhui Agricultural University, Hefei, 230036, China.
Youhua ZhangSchool of Information and Artificial Intelligence, Anhui Provincial Engineering Research Center for Beidou Precision Agriculture Information, Anhui Agricultural University, Hefei, 230036, China.
Yanjun ChenSchool of Agronomy, Anhui Agricultural University, Hefei, 230036, China.
Min LiSchool of Agronomy, Anhui Agricultural University, Hefei, 230036, China. twx6616@126.com.
Pengcheng WeiSchool of Agronomy, Anhui Agricultural University, Hefei, 230036, China. weipengcheng@gmail.com.
Zhenyu YueSchool of Information and Artificial Intelligence, Anhui Provincial Engineering Research Center for Beidou Precision Agriculture Information, Anhui Agricultural University, Hefei, 230036, China. zhenyuyue@ahau.edu.cn.ORCID http://orcid.org/0000-0002-9370-2540

Funding

National Natural Science Foundation of China 62102004National Natural Science Foundation of China 62472005National Natural Science Foundation of China U21A20214Open Fund of Anhui Provincial Engineering Laboratory for Beidou Precision Agriculture Information BDSY2023001
6 · The paper itself

Abstract

CRISPR/Cas base editors offer precise conversion of single nucleotides without inducing double-strand breaks. This technology finds extensive applications in gene therapy, gene function analysis, and other domains. However, a crucial challenge lies in selecting the appropriate guide RNAs (gRNAs) for base editing. Although various gRNAs design tools exist, creating a simplified base-editing library with diverse protospacer adjacent motifs (PAM) sequences for gRNAs screening remains a challenge. We present a user-friendly web tool, BES-Designer ( https://bes-designer.aielab.net ), for gRNAs design based on base editors, aimed at streamlining the creation of a base-editing library. BES-Designer incorporates our proposed rules for target sequence simplification, helping researchers narrow down the scope of biological experiments in the lab. It allows users to design target sequences with various PAMs and editing types simultaneously, and prioritize them in the simplified base-editing library. This tool has been experimentally proven to achieve a 30% simplification efficiency on the base-editing-library.

Indexed as

Gene EditingGene LibraryInternetRNA, Guide, CRISPR-Cas SystemsSoftwareCRISPR-Cas SystemsHumansRNA, Guide, CRISPR-Cas SystemsBase-editing-libraryCRISPR/CasGuide RNASimplification

Identifiers

PMID39466357

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.