Evidence map›Paper›PMID 39470208›Full record

ArticleJournal of virology2024

Structures of Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus virions reveal species-specific tegument and envelope features.

James Zhen, Jia Chen, Haigen Huang, Shiqing Liao, Shiheng Liu, Yan Yuan, Ren Sun, Richard Longnecker, Ting-Ting Wu, Z Hong Zhou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

James ZhenDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles (UCLA), Los Angeles, California, USA.ORCID 0000-0002-5163-835X
Jia ChenDepartment of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
Haigen HuangDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, UCLA, Los Angeles, California, USA.
Shiqing LiaoDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles (UCLA), Los Angeles, California, USA.
Shiheng LiuDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles (UCLA), Los Angeles, California, USA.
Yan YuanDepartment of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.ORCID 0000-0002-3064-1955
Ren SunDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, UCLA, Los Angeles, California, USA.
Richard LongneckerDepartment of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.ORCID 0000-0001-7175-6217
Ting-Ting WuDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, UCLA, Los Angeles, California, USA.
Z Hong ZhouDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles (UCLA), Los Angeles, California, USA.ORCID 0000-0002-8373-4717

Funding

Tumor Environment and Metastasis (TEAM) Research ProgramP30CA060553 · NCI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI Devalingam Mahalingam · 1993 to 2026
$153.9M
In situ atomic structures of the Kaposi's sarcoma-associated herpesvirus portal-terminase complex and glycoproteinsR01DE025567 · NIDCR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WU, TING-TING, ZHOU, Z HONG · 2016 to 2024
$4.0M
Interdisciplinary Training in Virology and Gene TherapyT32AI060567 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WU, TING-TING · 2004 to 2018
$3.4M
West/Midwest Consortium for High-Resolution Cryo Electron MicroscopyU24GM116792 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AGBANDJE-MCKENNA, MAVIS, DOKLAND, TERJE · 2016 to 2020
$3.0M
Roles of KSHV Tegument Proteins in Virion AssemblyR01DE027901 · NIDCR · UNIVERSITY OF PENNSYLVANIA · PI CHEN, CHI-DER · 2018 to 2022
$2.1M
HIGH-END CRYOEM INSTRUMENT FOR UCLA ELECTRON IMAGING CTR FOR NANOMACHINES: AIDSS10RR023057 · NCRR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHOU, Z HONG · 2006 to 2006
$1.6M
Direct Detection Device for atomic resolution cryoEM of macromolecular complexesS10OD018111 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHOU, Z HONG · 2014 to 2014
$598k
Epstein-Barr Virus Entry into Epithelial CellsR21AI175798 · NIAID · NORTHWESTERN UNIVERSITY AT CHICAGO · PI LONGNECKER, RICHARD M · 2024 to 2025
$430k
American Cancer Society (ACS) IRG-15-173-21HHS | National Institutes of Health (NIH) 5T32AI060567HHS | National Institutes of Health (NIH) R01DE025567HHS | National Institutes of Health (NIH) R01DE027901HHS | National Institutes of Health (NIH) R21AI175798NCI NIH HHS P30 CA060553NCRR NIH HHS S10 RR023057NIAID NIH HHS R21 AI175798NIAID NIH HHS T32 AI060567NIDCR NIH HHS R01 DE025567NIDCR NIH HHS R01 DE027901NIGMS NIH HHS U24 GM116792NIH HHS S10 OD018111
6 · The paper itself

Abstract

Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) are classified into the gammaherpesvirus subfamily of IMPORTANCE: Discovered in 1964, Epstein-Barr virus (EBV) is the first identified human oncogenic virus and the founding member of the gammaherpesvirus subfamily. In 1994, another cancer-causing virus was discovered in lesions of AIDS patients and later named Kaposi's sarcoma-associated herpesvirus (KSHV), the second human gammaherpesvirus. Despite the historical importance of EBV and KSHV, technical difficulties with isolating large quantities of these viruses and the pleiomorphic nature of their envelope and tegument layers have limited structural characterization of their virions. In this study, we employed the latest technologies in cryogenic electron microscopy (cryoEM) and tomography (cryoET) supplemented with an artificial intelligence-powered data processing software package to reconstruct 3D structures of the EBV and KSHV virions. We uncovered unique properties of the envelope glycoproteins and tegument layers of both EBV and KSHV. Comparison of these features with their non-tumorigenic counterparts provides insights into their relevance during infection.

Indexed as

Cryoelectron MicroscopyHerpesvirus 4, HumanHerpesvirus 8, HumanVirionCapsidElectron Microscope TomographyHumansNucleocapsidSpecies SpecificityViral Envelopeelectron microscopyEpstein-Barr virusglycoproteinsherpesviruseshuman herpesvirusesKaposi's sarcoma-associated herpesvirusvirion structure

Identifiers

PMID39470208
PMCPMC11575322

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.