Evidence map›Paper›PMID 39471168›Full record

ArticlePloS one2024

Identification of interaction partners of outer inflammatory protein A: Computational and experimental insights into how Helicobacter pylori infects host cells.

Sümeyye Akcelik-Deveci, Elif Kılıç, Nesteren Mansur-Ozen, Emel Timucin, Yaren Buyukcolak, Sinem Oktem-Okullu

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In one paragraph

Article in PloS one, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sümeyye Akcelik-DeveciDepartment of Medical Biotechnology, Institute of Health Sciences, Acibadem University, Atasehir, Istanbul, Turkey.
Elif KılıçDepartment of Medical Biotechnology, Institute of Health Sciences, Acibadem University, Atasehir, Istanbul, Turkey.
Nesteren Mansur-OzenDepartment of Medical Biotechnology, Institute of Health Sciences, Acibadem University, Atasehir, Istanbul, Turkey.
Emel TimucinDepartment of Biostatistics and Medical Informatics, School of Medicine, Acibadem University, Atasehir, Istanbul, Turkey.
Yaren BuyukcolakDepartment of Medical Biotechnology, Institute of Health Sciences, Acibadem University, Atasehir, Istanbul, Turkey.
Sinem Oktem-OkulluDepartment of Medical Microbiology, School of Medicine, Acibadem, Atasehir, Istanbul, Turkey.ORCID 0000-0002-2255-0930

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Outer membrane proteins (OMPs) play a key role in facilitating the survival of Helicobacter pylori within the gastric tissue by mediating adherence. Among these proteins, Outer inflammatory protein A (OipA) is a critical factor in H. pylori colonization of the host gastric epithelial cell surface. While the role of OipA in H. pylori attachment and its association with clinical outcomes have been established, the structural mechanisms underlying OipA's action in adherence to gastric epithelial cells remain limited. Our study employed experimental and computational approaches to investigate the interaction partners of OipA on the gastric epithelial cell surface. Initially, we conducted a proteomic analysis using a pull-down assay with recombinant OipA and gastric epithelial cell membrane proteins to identify the OipA interactome. This analysis revealed 704 unique proteins that interacted with OipA. We subsequently analyzed 16 of these OipA partners using molecular modeling tools. Among these 16 partners, we highlight three human proteins, namely Hepatocyte growth factor (HGF), Mesenchymal epithelial transition factor receptor (Met), and Adhesion G Protein-Coupled Receptor B1 (AGRB1) that could play a role in H. pylori adherence to the gastric epithelial cell surface with OipA. Collectively, these findings reveal novel host interactions mediated by OipA, suggesting their potential as therapeutic targets for combating H. pylori infection.

Indexed as

Bacterial Outer Membrane ProteinsEpithelial CellsHelicobacter InfectionsHelicobacter pyloriHepatocyte Growth FactorBacterial AdhesionGastric MucosaHost-Pathogen InteractionsHumansProtein BindingProteomicsProto-Oncogene Proteins c-metBacterial Outer Membrane ProteinsHepatocyte Growth FactorHGF protein, humanOipA protein, Helicobacter pyloriProto-Oncogene Proteins c-met

Identifiers

PMID39471168
PMCPMC11521304

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.