Evidence map›Paper›PMID 39473131›Full record

ArticleBritish journal of clinical pharmacology2026

Feasibility of dried blood spot collection for caffeine pharmacokinetic studies in microgravity: Insights from parabolic flight campaigns.

Audrey Derobertmasure, Li Shean Toh, Céline Verstuyft, Srboljub Lukic, Christelle De Sousa Carvalho, Raphaël Couronné, Marie Beauvalet, Stéphanie Chhun, Pierre Boutouyrie

Registry-linked trialAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in British journal of clinical pharmacology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06431984 (Kit de Pharmacologie Spatial - Analyse de l'Utilisation Des Dried Blood Spot Pour le Dosage de la caféine Chez Des Volontaires Sains en microgravité au Cours Des Vols Paraboliques), which is not on this map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT06431984 nacompletednot on this map

Kit de Pharmacologie Spatial - Analyse de l'Utilisation Des Dried Blood Spot Pour le Dosage de la caféine Chez Des Volontaires Sains en microgravité au Cours Des Vols Paraboliques

TypeinterventionalSponsorInstitut National de la Santé Et de la Recherche Médicale, FranceRan2023 to 2024Enrolled30ConditionsDrug Mechanism, Metabolism Medication Toxicity, Space Motion SicknessArmsprick test
3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Audrey DerobertmasureINSERM PARCC UMRS 970, Université Paris-Cité, Paris, France.ORCID https://orcid.org/0000-0002-7133-1400
Li Shean TohSchool of Pharmacy, Faculty of Science, University of Nottingham, University Park, Nottingham, UK.
Céline VerstuyftMolecular Genetics, Pharmacogenetics, and Hormonology Unit of Bicêtre, Paris-Saclay University Hospitals, Assistance Publique-Hôpitaux de Paris, Bicêtre Hospital, Paris, France.
Srboljub LukicLaboratory of Immunology and DMU BIOPHYGEN, Georges Pompidou European Hospital and Necker-enfants malades Hospital, AP-HP; Université Paris-Cité, INEM U1151, Paris, France.
Christelle De Sousa CarvalhoLaboratory of Immunology and DMU BIOPHYGEN, Georges Pompidou European Hospital and Necker-enfants malades Hospital, AP-HP; Université Paris-Cité, INEM U1151, Paris, France.
Raphaël CouronnéINSERM PARCC UMRS 970, Université Paris-Cité, Paris, France.
Marie BeauvaletINSERM PARCC UMRS 970, Université Paris-Cité, Paris, France.
Stéphanie ChhunLaboratory of Immunology and DMU BIOPHYGEN, Georges Pompidou European Hospital and Necker-enfants malades Hospital, AP-HP; Université Paris-Cité, INEM U1151, Paris, France.
Pierre BoutouyrieINSERM PARCC UMRS 970, Université Paris-Cité, Paris, France.

Funding

Centre National d'Etudes SpatialesInstitut National de la Santé et de la Recherche Médicale
6 · The paper itself

Abstract

aimsTherapeutic drug monitoring for astronauts faces limitations in conventional blood sampling and sample management onboard the international space station. Here, we explore the feasibility of dried blood spot (DBS) collection during parabolic flights (PF) to overcome these constraints.

methodsWe assessed the feasibility of blood deposition on blotting paper for preanalytical aspects in a PF using synthetic blood. Subsequently, DBS sampling validation was carried out in another PF campaign. Twenty volunteers participated in a pharmacokinetic study on caffeine and its metabolite, paraxanthine, conducted during parabolic flights. After >18 h caffeine washout, coffee (115 mg), tea (30 mg) or dark chocolate (11 mg) were ingested by the participants. DBS samples were collected at baseline, during weightlessness and post-flight. Caffeine and paraxanthine were analysed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Genotyping for cytochrome P450-1A2 (CYP1A2) was performed and a metabolic ratio by area under the curve for caffeine and paraxanthine (AUC

resultsFull in-flight pharmacokinetic study was feasible in seventeen volunteers with three unable to perform the sampling due to motion sickness. Nineteen participants (twelve males and seven females) completed pharmacokinetic profiles, with repeated pharmacokinetic studies for six participants. CYP1A2 genotyping resulted in eight ultrarapid, eleven intermediate, and one poor metabolizer. Among the women, four were on oestrogen contraceptives, a known inhibitor of CYP1A2, and were considered as poor metabolizers. Expected differences in kinetic profiles, consistent with consumption habits, the ingested dose and the genotypic/phenotypic information, were observed. The mean caffeine AUC for coffee, tea and chocolate were 9419 ng.h.mL

conclusionsDBS collection was safe, stable, feasible and well accepted in weightlessness. This method would offer valuable insights into human metabolism adaptation during long-term spaceflight, addressing space pharmacology challenges.

Indexed as

CaffeineDried Blood Spot TestingDrug MonitoringSpace FlightTheophyllineAdultArea Under CurveChocolateChromatography, LiquidCoffeeCytochrome P-450 CYP1A2Feasibility StudiesFemaleGenotypeHumansMaleCaffeineCoffeeCYP1A2 protein, humanCytochrome P-450 CYP1A2TeaTheophyllinedried blood spotmicrosamplingspace pharmacology

Identifiers

PMID39473131
PMCPMC12746359

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.