Evidence map›Paper›PMID 39479294›Full record

ArticleFrontiers in bioengineering and biotechnology2024

CRISPR-Cas9/Cas12a systems for efficient genome editing and large genomic fragment deletions in

Guoliang Yuan, Shuang Deng, Jeffrey J Czajka, Ziyu Dai, Beth A Hofstad, Joonhoon Kim, Kyle R Pomraning

Abstract read
In one paragraph

Article in Frontiers in bioengineering and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Review
  6. Advances in CRISPR/Cas9-Based Gene Editing in Filamentous Fungi.Journal of fungi (Basel, Switzerland) · 2025
    Review
  7. Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Guoliang YuanEnergy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, United States.
Shuang DengEnergy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, United States.
Jeffrey J CzajkaEnergy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, United States.
Ziyu DaiEnergy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, United States.
Beth A HofstadEnergy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, United States.
Joonhoon KimEnergy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, United States.
Kyle R PomraningEnergy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, United States.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

CRISPR technology has revolutionized fungal genetic engineering by accelerating the pace and expanding the feasible scope of experiments in this field. Among various CRISPR-Cas systems, Cas9 and Cas12a are widely used in genetic and metabolic engineering. In filamentous fungi, both Cas9 and Cas12a have been utilized as CRISPR nucleases. In this work we first compared efficacies and types of genetic edits for CRISPR-Cas9 and -Cas12a systems at the polyketide synthase (

Indexed as

Aspergillus nigerCas12aCRISPR-Cas9large fragment deletionsSquash-PCR

Identifiers

PMID39479294
PMCPMC11521959

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.