Evidence map›Paper›PMID 39480076›Full record

ArticlemSphere2024

The promiscuous biotin ligase TurboID reveals the proxisome of the T3SS chaperone IpgC in

Nathaline Haidar-Ahmad, Kyle Tomaro, Mathieu Lavallée-Adam, François-Xavier Campbell-Valois

Abstract read
In one paragraph

Article in mSphere, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Nathaline Haidar-AhmadDepartment of Chemistry and Biomolecular Sciences, Centre for Chemical and Synthetic Biology, Host-Microbe Interactions Laboratory, University of Ottawa, Ottawa, Ontario, Canada.
Kyle TomaroDepartment of Chemistry and Biomolecular Sciences, Centre for Chemical and Synthetic Biology, Host-Microbe Interactions Laboratory, University of Ottawa, Ottawa, Ontario, Canada.
Mathieu Lavallée-AdamDepartment of Biochemistry, Microbiology and Immunology, Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, Ontario, Canada.
François-Xavier Campbell-ValoisDepartment of Chemistry and Biomolecular Sciences, Centre for Chemical and Synthetic Biology, Host-Microbe Interactions Laboratory, University of Ottawa, Ottawa, Ontario, Canada.ORCID 0000-0001-5105-2968

Funding

Canadian Government | Canadian Institutes of Health Research (CIHR) 159517Canadian Government | Natural Sciences and Engineering Research Council of Canada (NSERC) RGPIN-2024-06723,RGPIN-2016-05587
6 · The paper itself

Abstract

Promiscuous biotin ligases derived from the bacterial enzyme BirA are used to identify proteins vicinal to a bait protein, thereby defining its proxisome. Despite the popularity of this approach, surprisingly little is known about its use in prokaryotes. Here, we compared the activity of four widely used promiscuous biotin ligases in the cytoplasm of

Indexed as

Bacterial ProteinsMolecular ChaperonesShigella flexneriType III Secretion SystemsBiotinCarbon-Nitrogen LigasesEscherichia coli ProteinsRepressor ProteinsBacterial ProteinsBiotinCarbon-Nitrogen LigasesEscherichia coli ProteinsMolecular ChaperonesRepressor ProteinsType III Secretion SystemsBioIDBirAcell signalingEnterobacteriaIpgCMxiEpromiscuous biotin ligaseprotein-protein interactionsShigella flexneritranscription regulationTurboIDtype III secretion system

Identifiers

PMID39480076
PMCPMC11580435

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.