ArticlePlant biotechnology journal2025
CRISPR/Cas knockout of the NADPH oxidase gene OsRbohB reduces ROS overaccumulation and enhances heat stress tolerance in rice.
Article in Plant biotechnology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Genome-Wide Identification and Analysis of Respiratory Burst Oxidase Homolog (International journal of molecular sciences · 2026Article
- Epigenetic regulation and ROS signaling in plant stress adaptation: an integrated framework.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Review
- Temperature regulation in plants: From molecular mechanisms to climate-resilient crop improvement.Journal of integrative plant biology · 2026Review
- Nudix Hydrolase as a Target for Regulating High Temperature Tolerance in Marine Diatoms.Microorganisms · 2026Article
- Knockout of OsRbohD (the NADPH Oxidase Gene) Enhances Saline-Alkaline Stress Tolerance and Grain Yield in Rice by Reducing ROS Accumulation.Rice (New York, N.Y.) · 2026Article
- Harnessing genomics and transcriptomics to explore the genetic and molecular basis of abiotic stress tolerance in minor millets - a comprehensive review.Functional & integrative genomics · 2026Review
- Applications of Gene-Editing Technologies in Enhancing Crop Stress Resistance with Emphasis on Rice.Plants (Basel, Switzerland) · 2026Review
- Fulvic Acid Suppresses Excessive Cytokinin Biosynthesis to Reduce ROS Content and thus Decrease NaRice (New York, N.Y.) · 2026Article
- Transcriptional reprogramming of heat-sensitive maize anther development under heat stress.BMC plant biology · 2026Article
- OsFeSOD3 Functions as an Enzymatic Component of the PEP Complex, Bifunctionally Regulating Chloroplastic ROS Metabolism and Chloroplast Biogenesis in Rice.Plant biotechnology journal · 2026Article
- Precision breeding in a changing climate: unlocking resilience through omics and gene editing.Functional & integrative genomics · 2026Review
- Review
- New progress in the production, oxidative damage, and scavenging mechanisms of reactive oxygen species in plants under abiotic stress.Frontiers in plant science · 2026Review
- Genetic and Molecular Basis for Heat Tolerance in Rice: Strategies for Resilience Under Climate Change.Plants (Basel, Switzerland) · 2025Review
- Rice Heat Stress Response: Physiological Changes and Molecular Regulatory Network Research Progress.Plants (Basel, Switzerland) · 2025Review
- CRISPR/Cas-Mediated Optimization of Soybean Shoot Architecture for Enhanced Yield.International journal of molecular sciences · 2025Review
- Integrated analysis of physiological responses and transcriptional regulation in kiwifruit under high temperature.BMC plant biology · 2025Article
- Effect of high temperature on pollen grains and yield in economically important crops: a review.Planta · 2025Review
- Rice Cytochrome P450 Protein CYP71P1 Is Required for Heat Stress Tolerance by Regulating Serotonin Biosynthesis and ROS Homeostasis.Plants (Basel, Switzerland) · 2025Article
- Decrypting molecular mechanism of heat stress tolerance in rice to tackle climate change challenges through recent approaches.Frontiers in plant science · 2025Review
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Authors and funding
11 authors.
Funding
Abstract
Heat stress (HS) has become a major factor limiting crop yields worldwide. HS inhibits plant growth by ROS accumulation, and NADPH oxidases (Rbohs) are major ROS producers in plants. Here, we show that CRISPR/Cas knockout of the OsRbohB (OsRbohB-KO) significantly increased rice tolerance to HS imposed at various different growth stages. We produced OsRbohB-KO and OsRbohB-overexpression (OsRbohB-OE) lines in a japonica cultivar, Nipponbare. Compared with nontransgenic wild-type (WT) plants, the OsRbohB-KO lines showed a significant increase in chlorophyll contents (5.2%-58.0%), plant growth (48.2%-65.6%) and grain yield (8.9%-20.5%), while reducing HS-induced ROS accumulation in seeds (21.3%-33.0%), seedlings (13.0%-30.4%), anthers (13.1%-20.3%) and grains (9.7%-22.1%), under HS conditions. Analysis of yield components revealed that the increased yield of OsRbohB-KO plants was due to increased starch synthetase activity, spikelets per panicle (2.0%-9.3%), filled spikelets (4.8%-15.5%), percentage of filled spikelets (2.4%-6.8%) and 1000-grain weight (2.9%-7.4%) under HS conditions during the reproductive stage. Grain milling and appearance quality, and starch content were also significantly increased in OsRbohB-KO plants under HS conditions during the mature stage. Furthermore, OsRbohB-KO significantly upregulated the expression levels of heat shock-related genes, OsHSP23.7, OsHSP17.7, OsHSF7 and OsHsfA2a, in rice seedlings and grains under long-term HS conditions. Conversely, OsRbohB-OE resulted in phenotypes that were opposite to OsRbohB-KO in most cases. Our results suggest that suppression of OsRbohB provides an effective approach for alleviating heat damage and improving grain yield and quality of rice under long-term HS conditions.
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