ArticleThe Plant journal : for cell and molecular biology2024
Fast and simple fluorometric measurement of phloem loading exposes auxin-dependent regulation of Arabidopsis sucrose transporter AtSUC2.
Article in The Plant journal : for cell and molecular biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- The loss of COP1 delays senescence and inhibits root growth through modulation of sucrose allocation and availability.Plant physiology · 2026Article
- siRNA-guided DNA-methylation at a sucrose transporter gene potentially contributes to climatic plant adaptation.PLoS biology · 2026Article
- Genome-Wide Identification and Characterization of thePlants (Basel, Switzerland) · 2026Article
- GeARF5/GeIAA33-GeSWEET14 module balances the secondary metabolic biosynthesis to increase the yield and quality in Gastrodia elata.Journal of integrative plant biology · 2026Article
- Regulatory DNA Sequences Controlling Gene Expression in Leaf Phloem Cells.Methods in molecular biology (Clifton, N.J.) · 2026Review
- A Dual-Reporter System for the Analysis of Phloem Structural and Signaling Responses In Vivo.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Predicting Phloem Transport Rate in Crops.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Measuring Esculin Export from Leaves as Proxy for Sucrose Loading Rates in the Phloem.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Nanotechnology Strategies in Plant Genetic Engineering: Intelligent Delivery and Precision Editing.Plants (Basel, Switzerland) · 2025Review
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Authors and funding
7 authors.
Funding
Abstract
The rate of sucrose export from leaves is a major factor in balancing whole-plant carbon and energy partitioning. A comprehensive study of its dynamics and relationship to photosynthesis, sink demand, and other relevant processes is hampered by the shortcomings of current methods for measuring sucrose phloem loading. We utilize the ability of sucrose transporter proteins, known as SUCs or SUTs, to specifically transport the fluorescent molecule esculin in a novel assay to measure phloem loading rates. Esculin was administered to source leaves and its fluorescence in the leaf extract was measured after 1 or 2 h. Dicot plants with an active phloem loading strategy showed an export-dependent reduction of esculin fluorescence. Relative leaf esculin export rates correlated with leaf export rates of isotopic carbon and phloem exudate sucrose levels. We used esculin experiments to examine the effects of phytohormones on phloem loading in Arabidopsis, showing, for example, that auxin induces phloem loading while cytokinin reduces it. Transcriptional regulation of AtSUC2 by AUXIN RESPONSE FACTOR1 (ARF1) corroborated the link between auxin signaling and phloem loading. Unlike established methods, the esculin assay is rapid and does not require specialized equipment. Potential applications and limitations of the esculin assay are discussed.
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Registered trials
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