Evidence map›Paper›PMID 39488203›Full record

ArticleStructure (London, England : 1993)2024

Structural and functional studies of the EGF20-27 region reveal new features of the human Notch receptor important for optimal activation.

Zhihan Bo, Thomas Rowntree, Steven Johnson, Hilman Nurmahdi, Richard J Suckling, Johan Hill, Boguslawa Korona, Philip C Weisshuhn, Devon Sheppard, Yao Meng and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Structure (London, England : 1993), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Zhihan BoDepartment of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Thomas RowntreeDepartment of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Steven JohnsonSir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
Hilman NurmahdiDepartment of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Richard J SucklingSir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
Johan HillDepartment of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Boguslawa KoronaDepartment of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Philip C WeisshuhnDepartment of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Devon SheppardSir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
Yao MengDepartment of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Shaoyan LiangDepartment of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Edward D LoweDepartment of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Susan M LeaSir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK. Electronic address: susan.lea@nih.gov.
Christina RedfieldDepartment of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. Electronic address: christina.redfield@bioch.ox.ac.uk.
Penny A HandfordDepartment of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. Electronic address: penny.handford@bioch.ox.ac.uk.

Funding

Medical Research Council MR/R009317/1Medical Research Council MR/V008935/1
6 · The paper itself

Abstract

The Notch receptor is activated by the Delta/Serrate/Lag-2 (DSL) family of ligands. The organization of the extracellular signaling complex is unknown, although structures of Notch/ligand complexes comprising the ligand-binding region (LBR), and negative regulatory region (NRR) region, have been solved. Here, we investigate the human Notch-1 epidermal growth factor-like (EGF) 20-27 region, located between the LBR and NRR, and incorporating the Abruptex (Ax) region, associated with distinctive Drosophila phenotypes. Our analyses, using crystallography, NMR and small angle X-ray scattering (SAXS), support a rigid, elongated organization for EGF20-27 with the EGF20-21 linkage showing Ca

Indexed as

Receptor, Notch1AnimalsBinding SitesCalciumCrystallography, X-RayEpidermal Growth FactorHumansJagged-1 ProteinModels, MolecularProtein BindingScattering, Small AngleX-Ray DiffractionCalciumEpidermal Growth FactorJagged-1 ProteinNOTCH1 protein, humanReceptor, Notch1

Identifiers

PMID39488203
PMCPMC12979247

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.