Evidence map›Paper›PMID 39488561›Full record

ArticleScientific reports2024

Hepatocyte growth factor upregulates MMP1 and MMP10 expression and resolves corneal fibrosis.

Mingshun Lee, Elsayed Elbasiony, Wonkyung J Cho, Vinay K Pulimamidi, Olufemi S Folorunso, Sharad K Mittal, Reza Dana, Sunil K Chauhan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mingshun LeeSchepens Eye Research Institute of Mass Eye & Ear, Harvard Medical School, 20 Staniford Street, Boston, MA, 02114, USA.
Elsayed ElbasionySchepens Eye Research Institute of Mass Eye & Ear, Harvard Medical School, 20 Staniford Street, Boston, MA, 02114, USA.
Wonkyung J ChoSchepens Eye Research Institute of Mass Eye & Ear, Harvard Medical School, 20 Staniford Street, Boston, MA, 02114, USA.
Vinay K PulimamidiSchepens Eye Research Institute of Mass Eye & Ear, Harvard Medical School, 20 Staniford Street, Boston, MA, 02114, USA.
Olufemi S FolorunsoSchepens Eye Research Institute of Mass Eye & Ear, Harvard Medical School, 20 Staniford Street, Boston, MA, 02114, USA.
Sharad K MittalSchepens Eye Research Institute of Mass Eye & Ear, Harvard Medical School, 20 Staniford Street, Boston, MA, 02114, USA.
Reza DanaSchepens Eye Research Institute of Mass Eye & Ear, Harvard Medical School, 20 Staniford Street, Boston, MA, 02114, USA.
Sunil K ChauhanSchepens Eye Research Institute of Mass Eye & Ear, Harvard Medical School, 20 Staniford Street, Boston, MA, 02114, USA. sunil_chauhan@meei.harvard.edu.

Funding

Ocular Immune Regulation by Mesenchymal Stem CellsR01EY024602 · NEI · SCHEPENS EYE RESEARCH INSTITUTE · PI CHAUHAN, SUNIL K · 2015 to 2025
$6.5M
NIH HHS R01EY024602US Department of Defense W81XWH2110962
6 · The paper itself

Abstract

Different therapeutic modalities, including steroids, have been used to treat corneal scarring. However, the ability of steroids to reduce corneal scarring is limited and associated with numerous side effects. Our previous studies have demonstrated that topical hepatocyte growth factor (HGF) after corneal injury suppresses the development of stromal scars. Here, we investigated whether HGF can re-establish corneal clarity and normalize tissue structure in corneas with pre-existing scars. Corneal scarring was induced by mechanically removing the corneal epithelium and the anterior third of the stroma using a hand-held Algerbrush II in C57BL/6 mice. Substantial scar tissue formed by day 10 post-injury, at which time the epithelium was debrided and treated with 0.1% recombinant mouse HGF, 0.1% dexamethasone (steroid) or 0.1% control protein thrice a day for 10 days. Corneal clarity was significantly restored in the HGF treatment group, compared to both the steroid and control protein treatment groups. Moreover, HGF treatment downregulated the expression of αSMA and upregulated the expression of extracellular matrix-remodeling matrix metalloproteinases 1 and 10 (MMP1 and MMP10), suggesting HGF upregulates tissue remodeling molecule MMP1 and 10 to promote tissue restoration. These findings offer novel insights into the mechanisms by which HGF re-establishes corneal clarity, and promotes epithelial regeneration in corneas with pre-existing stromal scarring.

Indexed as

CorneaFibrosisHepatocyte Growth FactorMatrix Metalloproteinase 1Matrix Metalloproteinase 10AnimalsCorneal DiseasesCorneal InjuriesDexamethasoneDisease Models, AnimalEpithelium, CornealMaleMiceMice, Inbred C57BLUp-RegulationDexamethasoneHepatocyte Growth FactorMatrix Metalloproteinase 1Matrix Metalloproteinase 10

Identifiers

PMID39488561
PMCPMC11531534

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.