Evidence mapPaperPMID 39497028Full record

ArticleBMC genomics2024

Genome wide identification and characterization of Bax inhibitor-1 gene family in cucumber (Cucumis sativus) under biotic and abiotic stress.

Samia Anwar, Riffat Siddique, Shakeel Ahmad, Muhammad Zeshan Haider, Haider Ali, Adnan Sami, Rosa Sanchez Lucas, Muhammad Shafiq, Bader Un Nisa, Bilal Javed and 3 more

Abstract read
In one paragraph

Article in BMC genomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Samia AnwarDepartment of Botany, Lahore College for Women University, P.O BOX. 54000, Lahore, Pakistan.
Riffat SiddiqueDepartment of Botany, Lahore College for Women University, P.O BOX. 54000, Lahore, Pakistan.
Shakeel AhmadSeed Center and Plant Genetic Resources Bank, Ministry of Environment, Water and Agriculture, Riyadh, 14712, Kingdom of Saudi Arabia.
Muhammad Zeshan HaiderDepartment of Plant Breeding and Genetics, Faculty of Agricultural Sciences, University of the Punjab, P.O BOX. 54590, Lahore, Pakistan.
Haider AliSchool of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
Adnan SamiDepartment of Plant Breeding and Genetics, Faculty of Agricultural Sciences, University of the Punjab, P.O BOX. 54590, Lahore, Pakistan.
Rosa Sanchez LucasSchool of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
Muhammad ShafiqDepartment of Horticulture, Faculty of Agricultural Sciences, University of the Punjab, P.O BOX. 54590, Lahore, Pakistan. shafiq.iags@pu.edu.pk.
Bader Un NisaDepartment of Botany, Lahore College for Women University, P.O BOX. 54000, Lahore, Pakistan.
Bilal JavedDepartment of Horticulture, Faculty of Agricultural Sciences, University of the Punjab, P.O BOX. 54590, Lahore, Pakistan.
Jannat AkramDepartment of Botany, Lahore College for Women University, P.O BOX. 54000, Lahore, Pakistan.
Javaria TabassumDepartment of Plant Breeding and Genetics, Faculty of Agricultural Sciences, University of the Punjab, P.O BOX. 54590, Lahore, Pakistan. javariatabassum.pbg@pu.edu.pk.
Muhammad Arshad JavedDepartment of Plant Breeding and Genetics, Faculty of Agricultural Sciences, University of the Punjab, P.O BOX. 54590, Lahore, Pakistan. chairman.pbg@pu.edu.pk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In plants, the BAX inhibitor-1 (BI-1) gene plays a crucial part in controlling cell death under stress conditions. This mechanism of Programmed Cell Death (PCD) is genetically regulated and is crucial for the elimination of unwanted or damaged cells in a controlled manner, which is essential for normal development and tissue maintenance. A study on cucumber identified and characterized five BI-1 genes: CsBI1, CsBI2, CsBI3, CsBI4, and CsBI5. These genes share conserved domains, indicating common evolutionary history and function. Physicochemical analysis revealed their molecular weights and isoelectric points, while subcellular localization showed their presence in different cellular compartments. The phylogenetic analysis highlighted evolutionary relationships with related crops. Chromosomal distribution and synteny analysis suggested segmental or tandem duplications within the gene family. Protein-protein interaction analysis revealed extensive interactions with other cucumber proteins. Cis-regulatory elements in the promoter regions provided insights into potential functions and transcriptional regulation. miRNAs showed diverse regulatory mechanisms, including mRNA cleavage and translational inhibition. The CsBI3, CsBI4 and CsBI5 genes exhibit elevated expression levels during cold stress, suggesting their vital involvement in cucumber plant defense mechanisms. The application of chitosan oligosaccharides externally confirms their distinct expression patterns. The qRT-PCR confirms the upregulation of CsBI genes in ToLCNDV-infected plants, indicating their potential to mitigate biotic and abiotic stresses. The comprehensive genome-wide exploration provides opportunities for the development of cold-tolerant and virus-resistant cucumber variants by traditional breeding or gene.

Indexed as

Cucumis sativusGene Expression Regulation, PlantMultigene FamilyPhylogenyPlant ProteinsStress, PhysiologicalChromosomes, PlantGenome, PlantMicroRNAsPromoter Regions, GeneticSyntenyMicroRNAsPlant ProteinsAbiotic stressBAX inhibitor-1BioinformaticsBiotic stressCa+ 2 levelCucumis sativusER stress

Identifiers

PMID39497028
PMCPMC11536926

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.