Evidence map›Paper›PMID 39497200›Full record

ArticleEuropean journal of medical research2024

Electroacupuncture alleviated post-stroke cognitive impairment via the mTOR/NLRP3-mediated autophagy-inflammatory pathway.

Jiawang Lang, Jianchang Luo, Jiachen Lang, Luodan Wang, Wenbin Xu, Jie Jia, Zhipeng Zhao, Boxu Lang

Abstract read
In one paragraph

Article in European journal of medical research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Trial
  3. Article
  4. Article
  5. Article
  6. Article
  7. [Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2026
    Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jiawang Lang *Department of Rehabilitation Medicine, Taizhou Municipal Hospital, No.381-1, Zhongshan East Road, Taizhou, 318000, Zhejiang, China.
Jianchang Luo *Department of Rehabilitation Medicine, Taizhou Municipal Hospital, No.381-1, Zhongshan East Road, Taizhou, 318000, Zhejiang, China.
Jiachen LangDepartment of Acupuncture and Massage Department, Acupuncture and Massage College, Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, Guizhou, China.
Luodan WangDepartment of Rehabilitation Medicine, Taizhou Municipal Hospital, No.381-1, Zhongshan East Road, Taizhou, 318000, Zhejiang, China.
Wenbin XuDepartment of Rehabilitation Medicine, Taizhou Municipal Hospital, No.381-1, Zhongshan East Road, Taizhou, 318000, Zhejiang, China.
Jie JiaDepartment of Rehabilitation Medicine, Huashan Hospital Affiliated to Fudan University, Shanghai, 200000, China.
Zhipeng ZhaoDepartment of Rehabilitation Medicine, School of Medicine, Taizhou University, Taizhou, 318000, Zhejiang, China.
Boxu LangDepartment of Rehabilitation Medicine, Taizhou Municipal Hospital, No.381-1, Zhongshan East Road, Taizhou, 318000, Zhejiang, China. tsylbx@tzc.edu.cn.

Funding

2022 Zhejiang provincial public welfare Technology Application Research Funded Project LGF22H270001Construction project of inheritance studio of famous and old TCM experts in Zhejiang Province in 2020 GZS2020049
6 · The paper itself

Abstract

backgroundPost-stroke cognitive impairment (PSCI) severely reduces quality of life of patients with stroke. This study aimed to assess the effects of electroacupuncture (EA) on PSCI and the role of the mTOR/NLRP3-mediated autophagy-inflammatory pathway in this process.

methodsThe rat focal cerebral ischemia model was established using middle cerebral artery occlusion (MCAO). Following successful induction of the model, EA was applied to the bilateral Fengchi, Fengfu, and Dazhui acupoints, and brain tissue samples were collected on day 15. Cognitive function was assessed using the Morris water maze test. Cerebral infarct volume was quantified by Triphenyltetrazolium chloride (TTC) staining. Hematoxylin-eosin and TUNEL staining were performed to evaluate pathological changes and apoptosis rates. Apoptosis-, inflammation-, and autophagy-related biomarkers were measured, and autophagosomes were visualized using transmission electron microscopy.

resultsMCAO rats exhibited slower weight gain, reduced mobility, increased infarct size, pathological damage, and apoptosis, confirming successful establishment of the MCAO rat model. Following EA treatment, MCAO rats displayed faster weight gain, improved mobility, and shorter escape latency. EA also reduced the area of cerebral infarction and alleviated pathological damage and apoptosis in MCAO rats. Furthermore, EA downregulated IL-1β, IL-18, NLRP3, and LC3 II/LC3 I expression and upregulated p62, mTOR, and Beclin-1 expression in MCAO rats. EA treatment also decreased the number of autophagosomes in these rats.

conclusionsEA effectively mitigates post-stroke cognitive impairment by reducing apoptosis, inflammation, and autophagy through the regulation of the mTOR/NLRP3-mediated autophagy-inflammatory pathway, offering valuable therapeutic insights for stroke rehabilitation.

Indexed as

AutophagyCognitive DysfunctionElectroacupunctureNLR Family, Pyrin Domain-Containing 3 ProteinRats, Sprague-DawleyTOR Serine-Threonine KinasesAnimalsApoptosisDisease Models, AnimalInfarction, Middle Cerebral ArteryInflammationMaleRatsSignal TransductionStrokemTOR protein, ratNLR Family, Pyrin Domain-Containing 3 ProteinNlrp3 protein, ratTOR Serine-Threonine KinasesApoptosisAutophagyInflammationmTOR/NLRP3 pathwayPost-stroke cognitive impairment

Identifiers

PMID39497200
PMCPMC11536957

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.