Evidence map›Paper›PMID 39501289›Full record

ArticleVirology journal2024

A novel neutralizing monoclonal antibody recognizes a linear antigenic epitope of the spike protein of swine acute diarrhoea syndrome coronavirus.

Lin Zhang, Hui-Zhen Liu, Yuexiao Lian, Yujun Zhu, Miaoli Wu, Jianbo Liu, Feng Cong

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Lin Zhang *Guangdong Laboratory Animals Monitoring Institute, Guangzhou, 510663, PR China.
Hui-Zhen Liu *Guangdong Laboratory Animals Monitoring Institute, Guangzhou, 510663, PR China.
Yuexiao LianGuangdong Laboratory Animals Monitoring Institute, Guangzhou, 510663, PR China.
Yujun ZhuGuangdong Laboratory Animals Monitoring Institute, Guangzhou, 510663, PR China.
Miaoli WuGuangdong Laboratory Animals Monitoring Institute, Guangzhou, 510663, PR China.
Jianbo LiuState Key Laboratory of Respiratory Disease, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong Provincial Key Laboratory of Biocomputing, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, PR China. liu_jianbo@gibh.ac.cn.
Feng CongGuangdong Laboratory Animals Monitoring Institute, Guangzhou, 510663, PR China. congfeng521@126.com.

Funding

National Key Research and Development Program of China 2021YFF0703300
6 · The paper itself

Abstract

Swine acute diarrhoea syndrome coronavirus (SADS-CoV) causes vomiting, severe diarrhoea and death in newborn piglets. The spike (S) protein plays a crucial role in promoting virus invasion and inducing neutralizing antibody production. In this study, the extracellular region of the S protein was used as an immunogen to immunize BALB/c mice. After immunization, B cells were collected, fused with SP2/0 myeloma cells, cultured and subcloned, and a cell line capable of secreting neutralizing antibodies was obtained and named as 5D6. Additionally, it was determined that the 5D6 mAb could be used as the primary antibody for western blotting and indirect immunofluorescence assay (IFA) to detect SADS-CoV. Further studies indicated that the 5D6 mAb binds to the

Indexed as

Antibodies, MonoclonalAntibodies, NeutralizingAntibodies, ViralEpitopesMice, Inbred BALB CSpike Glycoprotein, CoronavirusAlphacoronavirusAnimalsCoronavirus InfectionsEpitope MappingMiceSwineSwine DiseasesAntibodies, MonoclonalAntibodies, NeutralizingAntibodies, ViralEpitopesSpike Glycoprotein, CoronavirusAntigenic epitopeNeutralizing antibodiesSwine acute diarrhoea syndrome coronavirus (SADS-CoV)

Identifiers

PMID39501289
PMCPMC11539425

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.