Evidence mapPaperPMID 39504361Full record

ArticleScience advances2024

Endosomes serve as signaling platforms for RIG-I ubiquitination and activation.

Kuan-Ru Chen, Chia-Yu Yang, San-Ging Shu, Yin-Chiu Lo, Kuan-Wei Lee, Li-Chun Wang, Jia-Bao Chen, Meng-Cen Shih, Hung-Chun Chang, Yu-Ju Hsiao and 3 more

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Article in Science advances, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Kuan-Ru ChenDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University, 70101 Tainan, TaiwanORCID 0000-0003-1132-4492
Chia-Yu YangImmunology Research Center, National Health Research Institutes, Zhunan, Taiwan.ORCID 0000-0002-5123-0856
San-Ging ShuDepartment of Pediatrics, Ditmanson Medical Foundation Chia-Yi Christian Hospital, 60002 Chiayi City, Taiwan.
Yin-Chiu LoDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University, 70101 Tainan, Taiwan
Kuan-Wei LeeDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University, 70101 Tainan, Taiwan
Li-Chun WangDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University, 70101 Tainan, Taiwan
Jia-Bao ChenDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University, 70101 Tainan, Taiwan
Meng-Cen ShihDepartment of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, 70101 Tainan, Taiwan.
Hung-Chun ChangDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University, 70101 Tainan, Taiwan
Yu-Ju HsiaoDepartment of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, 70101 Tainan, Taiwan.
Chao-Liang WuDepartment of Medical Research, Ditmanson Medical Foundation Chia-Yi Christian Hospital, 60002 Chiayi City, Taiwan.ORCID 0000-0001-7821-9406
Tse-Hua TanImmunology Research Center, National Health Research Institutes, Zhunan, Taiwan.ORCID 0000-0003-4969-3170
Pin LingDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University, 70101 Tainan, TaiwanORCID 0000-0002-8894-5433

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

RIG-I-like receptors (RLRs) are cytosolic RNA sensors critical for antiviral immunity. RLR activation is regulated by polyubiquitination and oligomerization following RNA binding. Yet, little is known about how RLRs exploit subcellular organelles to facilitate their posttranslational modifications and activation. Endosomal adaptor TAPE regulates the endosomal TLR and cytosolic RLR pathways. The potential interplay between RIG-I signaling and endosomes has been explored. Here, we report that endosomes act as platforms for facilitating RIG-I polyubiquitination and complex formation. RIG-I was translocated onto endosomes to form signaling complexes upon activation. Ablation of endosomes impaired RIG-I signaling to type I IFN activation. TAPE mediates the interaction and polyubiquitination of RIG-I and TRIM25. TAPE-deficient myeloid cells were defective in type I IFN activation upon RNA ligand and virus challenges. Myeloid TAPE deficiency increased the susceptibility to RNA virus infection in vivo. Our work reveals endosomes as signaling platforms for RIG-I activation and antiviral immunity.

Indexed as

DEAD Box Protein 58EndosomesSignal TransductionUbiquitinationAnimalsHEK293 CellsHumansInterferon Type IMiceTripartite Motif ProteinsUbiquitin-Protein LigasesDEAD Box Protein 58Interferon Type ITripartite Motif ProteinsUbiquitin-Protein Ligases

Identifiers

PMID39504361
PMCPMC11540011

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.