Evidence map›Paper›PMID 39519212›Full record

ArticleInternational journal of molecular sciences2024

Phenotypic Biomarkers of Aqueous Extracellular Vesicles from Retinoblastoma Eyes.

Anne Amacker, Chen-Ching Peng, Nan Jiang, Shreya Sirivolu, Nikki Higa, Kevin Stachelek, Bibiana Reiser, Peter Kuhn, David Cobrinik, Paolo Neviani and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Recent advances in biomarkers for cardiac fibrosis.Frontiers in cardiovascular medicine · 2026
    Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Anne AmackerThe Vision Center, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.ORCID 0009-0006-0801-6607
Chen-Ching PengThe Vision Center, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
Nan JiangDepartment of Cancer Biology and Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, CA 91010, USA.ORCID 0009-0009-5976-8042
Shreya SirivoluThe Vision Center, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.ORCID 0009-0007-5871-9084
Nikki HigaMichelson Center for Convergent Bioscience, Convergent Science Institute in Cancer, University of Southern California, Los Angeles, CA 90089, USA.ORCID 0000-0003-3236-8929
Kevin StachelekThe Vision Center, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
Bibiana ReiserThe Vision Center, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
Peter KuhnMichelson Center for Convergent Bioscience, Convergent Science Institute in Cancer, University of Southern California, Los Angeles, CA 90089, USA.ORCID 0000-0003-2629-4505
David CobrinikThe Vision Center, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
Paolo NevianiThe Extracellular Vesicle Core, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
Jesse L BerryThe Vision Center, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.ORCID 0000-0002-8907-9100
Tijana Jovanovic-TalismanDepartment of Cancer Biology and Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, CA 91010, USA.ORCID 0000-0003-1928-4763
Liya XuThe Vision Center, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.ORCID 0000-0002-5501-2039

Funding

Ophthalmic Therapeutics Engineering CoreP30EY029220 · NEI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Mahnaz Shahidi · 2018 to 2026
$6.5M
Molecular dissection and imaging of extracellular vesicles to define their origin and targetsUH3TR002878 · NCATS · MASSACHUSETTS GENERAL HOSPITAL · PI DAS, SAUMYA, TALISMAN, TIJANA · 2021 to 2022
$2.2M
Molecular dissection and imaging of extracellular vesicles to define their origin and targetsUG3TR002878 · NCATS · MASSACHUSETTS GENERAL HOSPITAL · PI DAS, SAUMYA, TALISMAN, TIJANA · 2019 to 2020
$939k
Children's Hospital Los Angeles Saban Research Institute Research Career Development AwardMiriam and Sheldon G. Adelson Medical Research Foundation N/ANCATS NIH HHS UG3 TR002878NEI NIH HHS P30 EY029220NIH HHS UG3/UH3 TR002878The Knights Templar Eye Foundation N/AUnrestricted Grant to the Department of Ophthalmology from Research to Prevent Blindness N/AUSC Dean's Pilot N/A
6 · The paper itself

Abstract

Recent advancements in aqueous humor (AH) cell-free DNA (cfDNA) genomics have opened new avenues for ex vivo molecular profiling of retinoblastoma (RB), the most common pediatric intraocular malignancy, where biopsy is typically prohibited. While these insights offer a genetic blueprint of the tumor, they lack multi-omic molecular phenotyping, which is essential for understanding the functional state. Extracellular vesicles (EVs), naturally present in AH, are promising by offering time-resolved phenotypic information. We employed multiplex bead-based flow cytometry and Single Extracellular Vesicle Nanoscopy (SEVEN) to analyze EV phenotypes in AH from a cohort of five RB, with three uveal melanoma (UM) and two age-matched glaucoma (GLC) samples serving as controls. The studies identified CD133-enriched EVs uniquely in RB AH, absent in both GLC and UM AH. This was corroborated by further analysis of five RB cell lines, including two commercial (Y79, Weri) and three in-house developed lines, confirming CD133 enrichment and supporting its role as an RB-specific EV marker. Single-vesicle analysis demonstrated a strong association of CD133 with CD81 and CD63, with minimal CD9 presence. These results, validated through complementary techniques, position CD133 as a critical marker in RB-derived EVs, paving the way for enhanced multi-omic RB characterization and potential advancements in clinical diagnostics.

Indexed as

AC133 AntigenAqueous HumorBiomarkers, TumorExtracellular VesiclesRetinoblastomaCell Line, TumorChildChild, PreschoolFemaleGlaucomaHumansInfantMaleMelanomaPhenotypeRetinal NeoplasmsAC133 AntigenBiomarkers, TumorCD63 protein, humanCD81 protein, humanCD9 protein, humanPROM1 protein, humanTetraspanin 28Tetraspanin 29Tetraspanin 30aqueous humorCD133extracellular vesiclesmolecular phenotypingretinoblastoma

Identifiers

PMID39519212
PMCPMC11545953

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.