Bacterial expression, purification, and characterization of human cytochrome P450 3A4 without N-terminal modifications.

Yudong Sun, Yoichi Osawa, Haoming Zhang

Abstract read

In one paragraph

Article in Archives of biochemistry and biophysics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

High-level expression of full-length human CYP2E1 in Escherichia coli: Comparison of full-length human CYP2E1 to truncated counterpart shows enhanced association with redox partners.Drug metabolism and disposition: the biological fate of chemicals · 2026
Article
A Novel Technology Platform for Extracellular Vesicle-Targeted Expression of Drug-Metabolizing Enzymes: Driving CYP3A4 Expression and Secretion via the EABR Motif.Biomedicines · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Yudong SunDepartment of Pharmacology, The University of Michigan Medical School, Ann Arbor, MI, 48109, USA.

Yoichi OsawaDepartment of Pharmacology, The University of Michigan Medical School, Ann Arbor, MI, 48109, USA.

Haoming ZhangDepartment of Pharmacology, The University of Michigan Medical School, Ann Arbor, MI, 48109, USA. Electronic address: haom@umich.edu.

Funding

P450 and NO Synthase Regulation by Multiprotein ComplexesR01GM077430 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI OSAWA, YOICHI, ZHANG, HAOMING · 2006 to 2022

$6.5M

NIGMS NIH HHS R01 GM077430

6 · The paper itself

Abstract

In this communication we reported a bacterial system that over-expressed full-length wild-type (WT) human CYP3A4 in Escherichia coli (E. coli) at a level of 495 nmol/L culture. This level of expression was achieved by cloning the cDNA sequence of CYP3A4 WT to a pLW01-P450 vector and co-expressing it with chaperones GroEL/ES in bacterial C41(DE3) cells. Aided with a C-terminal His

Indexed as

Cytochrome P-450 CYP3AEscherichia coliChaperonin 60Cloning, MolecularGene ExpressionHumansRecombinant ProteinsTestosteroneChaperonin 60CYP3A4 protein, humanCytochrome P-450 CYP3ARecombinant ProteinsTestosteroneHeterologous expressionP450PurificationTestosterone hydroxylationWild-type CYP3A4

Identifiers

PMID39522857

DOI10.1016/j.abb.2024.110208

PMCPMC13256603

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.