Evidence map›Paper›PMID 39523091›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2024

[Nlrp6 overexpression inhibits lipid synthesis to suppress proliferation of hepatocellular carcinoma cells by regulating the AMPK-Srebp1c axis].

C Huang, Y Sun, W Li, L Liu, W Wang, J Zhang

Abstract readEnglish Abstract
PubMed Full text at PMC Publisher
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Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

C HuangCentral Laboratory, First College of Clinical Medical Science of China Three Gorges University (Yichang Central People's Hospital)//Hubei Key Laboratory of Ischemic Cardiovascular Disease//Hubei Provincial Clinical Research Center for Ischemic Cardiovascular Disease, Yichang 443003, China.
Y SunCentral Laboratory, First College of Clinical Medical Science of China Three Gorges University (Yichang Central People's Hospital)//Hubei Key Laboratory of Ischemic Cardiovascular Disease//Hubei Provincial Clinical Research Center for Ischemic Cardiovascular Disease, Yichang 443003, China.
W LiCentral Laboratory, First College of Clinical Medical Science of China Three Gorges University (Yichang Central People's Hospital)//Hubei Key Laboratory of Ischemic Cardiovascular Disease//Hubei Provincial Clinical Research Center for Ischemic Cardiovascular Disease, Yichang 443003, China.
L LiuCentral Laboratory, First College of Clinical Medical Science of China Three Gorges University (Yichang Central People's Hospital)//Hubei Key Laboratory of Ischemic Cardiovascular Disease//Hubei Provincial Clinical Research Center for Ischemic Cardiovascular Disease, Yichang 443003, China.
W WangCentral Laboratory, First College of Clinical Medical Science of China Three Gorges University (Yichang Central People's Hospital)//Hubei Key Laboratory of Ischemic Cardiovascular Disease//Hubei Provincial Clinical Research Center for Ischemic Cardiovascular Disease, Yichang 443003, China.
J ZhangCentral Laboratory, First College of Clinical Medical Science of China Three Gorges University (Yichang Central People's Hospital)//Hubei Key Laboratory of Ischemic Cardiovascular Disease//Hubei Provincial Clinical Research Center for Ischemic Cardiovascular Disease, Yichang 443003, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the mechanism of Nlrp6 for regulating hepatocellular carcinoma (HCC) progression in light of lipid synthesis regulation.

methodsNlrp6 expression level in HCC tissues of different pathological grades was investigated using RNA-seq data from The Cancer Genome Atlas (TCGA) database, and its correlation with the patients' survival was analyzed with Kaplan-Meier survival analysis. HepG2 cells with adenovirus-mediated Nlrp6 overexpression or knockdown were treated with palmitic acid (PA), and the changes in lipid deposition and cell proliferation were evaluated using Oil Red O staining, CCK-8 assay, EdU staining, and colony formation assay. RT-qPCR and Western blotting were used to detect the changes in expression of lipid synthesis-related genes and the proteins in the AMPK-Srebp1c axis. In a mouse model of hepatic steatosis established in liver-specific Nlrp6 knockout mice by high-fat diet feeding for 24 weeks, liver fibrosis was examined with histological staining, and the changes in expressions of HCC markers and the AMPK-Srebp1c signaling pathway were detected.

resultsNlrp6 expression was significantly reduced in HCC tissues with negative correlations with the pathological grades and the patients' survival (

conclusionNlrp6 overexpression inhibits lipid synthesis in HCC cells by regulating the AMPK-Srebp1c axis, which might be a key pathway for suppressing HCC cell proliferation.

Indexed as

AMP-Activated Protein KinasesCarcinoma, HepatocellularCell ProliferationLiver NeoplasmsSterol Regulatory Element Binding Protein 1AnimalsHep G2 CellsHumansLipid MetabolismLipidsMiceMice, KnockoutSignal TransductionAMP-Activated Protein KinasesLipidsSREBF1 protein, humanSterol Regulatory Element Binding Protein 1hepatic steatosishepatocellular carcinoma cellslipid synthesisNlrp6proliferation

Identifiers

PMID39523091
PMCPMC11526467

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.