Evidence mapPaperPMID 39532098Full record

ArticleMolecular therapy : the journal of the American Society of Gene Therapy2024

Efficient and selective kidney targeting by chemically modified carbohydrate conjugates.

Vikas Kumar, Aniket Wahane, Ming Shen Tham, Stefan Somlo, Anisha Gupta, Raman Bahal

Abstract read
In one paragraph

Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Review
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  6. Article
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  9. Review
  10. Not all carbs are bad for the kidney.Molecular therapy : the journal of the American Society of Gene Therapy · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Vikas KumarDepartment of Pharmaceutical Sciences, University of Connecticut, Storrs, CT 06269, USA.
Aniket WahaneDepartment of Pharmaceutical Sciences, University of Connecticut, Storrs, CT 06269, USA.
Ming Shen ThamDepartment of Internal Medicine, Yale School of Medicine, New Haven, CT 06520, USA.
Stefan SomloDepartment of Internal Medicine, Yale School of Medicine, New Haven, CT 06520, USA; Department of Genetics, Yale School of Medicine, New Haven, CT 06520, USA.
Anisha GuptaSchool of Pharmacy, University of Saint Joseph, West Hartford, CT 06117, USA.
Raman BahalDepartment of Pharmaceutical Sciences, University of Connecticut, Storrs, CT 06269, USA. Electronic address: raman.bahal@uconn.edu.

Funding

Yale Clinical and Translational Science AwardUL1TR001863 · YALE UNIVERSITY · 2025 to 2025
$9.9M
NCATS NIH HHS UL1 TR001863
6 · The paper itself

Abstract

We investigated a renal tubule-targeting carbohydrate (RENTAC) that can selectively deliver small-molecule and nucleic acid analogs to the proximal convoluted tubules of the kidney following systemic delivery in mice. We comprehensively evaluated anti-miR-21-peptide nucleic acid-RENTAC, and fluorophore-RENTAC conjugates in cell culture and in vivo. We established that RENTAC conjugates showed megalin- and cubilin-dependent endocytic uptake in the immortalized kidney cell line. In vivo biodistribution studies confirmed the retention of RENTAC conjugates in the kidneys for several days compared with other organs. Immunofluorescence staining confirmed the selective distribution of the RENTAC conjugates in proximal convoluted tubules. We further demonstrated proximal convoluted tubule targeting features of RENTAC conjugates in a folic acid-induced kidney fibrosis mouse model. As a biological readout, we targeted miR-33 using antisense peptide nucleic acid (PNA) 33-RENTAC conjugates in the fibrotic kidney disease model. The targeted delivery of PNA 33-RENTAC resulted in slower fibrosis progression and decreased collagen deposition. We also confirmed that the RENTAC ligand did not exert any adverse reactions. Thus, we established that the RENTAC ligand can be used for broad clinical applications targeting the kidneys selectively.

Indexed as

MicroRNAsPeptide Nucleic AcidsAnimalsCarbohydratesCell LineDisease Models, AnimalFibrosisFolic AcidHumansKidneyKidney DiseasesKidney Tubules, ProximalLow Density Lipoprotein Receptor-Related Protein-2MiceReceptors, Cell SurfaceTissue DistributionCarbohydratesFolic Acidintrinsic factor-cobalamin receptorLow Density Lipoprotein Receptor-Related Protein-2MicroRNAsMIRN21 microRNA, mousePeptide Nucleic AcidsReceptors, Cell Surfaceacetylated lactobionic acidantisense oligonucleotidecubilinfibrosiskidney deliverymegalinmiR-33peptide nucleic acidproximal convoluted tubulesRENTAC

Identifiers

PMID39532098
PMCPMC11638880

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.