Evidence map›Paper›PMID 39535501›Full record

ReviewChemical reviews2024

Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.

Anneliese M M Gest, Ayse Z Sahan, Yanghao Zhong, Wei Lin, Sohum Mehta, Jin Zhang

Abstract readReview
In one paragraph

Review in Chemical reviews, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed.

  1. Article
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  15. Inorganic phosphate and the rapid mobilization of metabolic energy in neurons.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Anneliese M M GestDepartment of Pharmacology, University of California, San Diego, La Jolla, California 92093, United States.ORCID 0000-0002-0923-2989
Ayse Z SahanDepartment of Pharmacology, University of California, San Diego, La Jolla, California 92093, United States.
Yanghao ZhongDepartment of Pharmacology, University of California, San Diego, La Jolla, California 92093, United States.
Wei LinDepartment of Pharmacology, University of California, San Diego, La Jolla, California 92093, United States.ORCID 0000-0002-2128-241X
Sohum MehtaDepartment of Pharmacology, University of California, San Diego, La Jolla, California 92093, United States.ORCID 0000-0003-4764-8579
Jin ZhangDepartment of Pharmacology, University of California, San Diego, La Jolla, California 92093, United States.ORCID 0000-0001-7145-7823

Funding

UCSD/SDSU IRACDAK12GM068524 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI TREJO, JOANN · 2003 to 2025
$24.4M
Live-cell Activity Architecture in CancerR35CA197622 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jin Zhang · 2015 to 2026
$11.2M
Mechanisms of Compartmentalized cAMP SignalingR01DK073368 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jin Zhang · 2006 to 2026
$7.6M
Blood Cells in Hemostasis and ThrombosisT32HL086344 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GINSBERG, MARK HOWARD · 2008 to 2023
$4.4M
Ultrasensitive kinase biosensors for multiplex imaging of coordinated spatiotemporal signaling in cancer-immune interactionsR01CA262815 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Yingxiao Wang, Jin Zhang · 2022 to 2026
$3.3M
AMPK Regulation of ACE2 in Endothelial Health and DiseaseR01HL162302 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SHYY, JOHN YJ, ZHANG, JIN · 2022 to 2025
$2.7M
Development of kinase biosensors for multiplex neuronal imaging of signaling pathways in behaving miceRF1MH126707 · NIMH · JOHNS HOPKINS UNIVERSITY · PI HUGANIR, RICHARD L, ZHANG, JIN · 2022 to 2022
$2.4M
Signal Transduction by PI3K/mTORR01DE030497 · NIDCR · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GUTKIND, JORGE SILVIO, ZHANG, JIN · 2021 to 2025
$2.4M
Spatial regulation of mechanistic target of rapamycin complex 1 (mTORC1) and its role in oral squamous cell carcinomaF31DE032886 · NIDCR · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SAHAN, AYSE ZISAN · 2023 to 2024
$85k
NCI NIH HHS R01 CA262815NCI NIH HHS R35 CA197622NHLBI NIH HHS R01 HL162302NHLBI NIH HHS T32 HL086344NIDCR NIH HHS F31 DE032886NIDCR NIH HHS R01 DE030497NIDDK NIH HHS R01 DK073368NIGMS NIH HHS K12 GM068524NIMH NIH HHS RF1 MH126707
6 · The paper itself

Abstract

Cellular function is controlled through intricate networks of signals, which lead to the myriad pathways governing cell fate. Fluorescent biosensors have enabled the study of these signaling pathways in living systems across temporal and spatial scales. Over the years there has been an explosion in the number of fluorescent biosensors, as they have become available for numerous targets, utilized across spectral space, and suited for various imaging techniques. To guide users through this extensive biosensor landscape, we discuss critical aspects of fluorescent proteins for consideration in biosensor development, smart tagging strategies, and the historical and recent biosensors of various types, grouped by target, and with a focus on the design and recent applications of these sensors in living systems.

Indexed as

Biosensing TechniquesLuminescent ProteinsAnimalsFluorescent DyesHumansSignal TransductionFluorescent DyesLuminescent Proteins

Identifiers

PMID39535501
PMCPMC11613326

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.