ArticlebioRxiv : the preprint server for biology2024
Endogenous EWSR1-FLI1 degron alleles enable control of fusion oncoprotein expression in tumor cell lines and xenografts.
James H McGinnis, Alberto Bremauntz Enriquez, Florentina Vandiver, Xin Bai, Jiwoong Kim, Jessica Kilgore, Purbita Saha, Ryan O'Hara, Yang Xie, Laura A Banaszynski and 2 more
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In one paragraphArticle in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 · Who and what moneyAuthors and funding
12 authors.
James H McGinnisDepartment of Internal Medicine, Division of Endocrinology, University of Texas Southwestern Medical Center, Dallas, TX 75390 USA.
Alberto Bremauntz EnriquezDepartment of Internal Medicine, Division of Endocrinology, University of Texas Southwestern Medical Center, Dallas, TX 75390 USA.ORCID 0000-0002-1216-3099 Florentina VandiverDepartment of Internal Medicine, Division of Endocrinology, University of Texas Southwestern Medical Center, Dallas, TX 75390 USA.
Xin BaiDepartment of Internal Medicine, Division of Endocrinology, University of Texas Southwestern Medical Center, Dallas, TX 75390 USA.
Jiwoong KimQuantitative Biomedical Research Center, Peter O'Donnell School of Public Health, University of Texas Southwestern Medical Center, Dallas, TX 75390 USA.
Jessica KilgoreDepartment of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390 USA.
Purbita SahaCecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390 USA.
Ryan O'HaraCecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390 USA.
Yang XieQuantitative Biomedical Research Center, Peter O'Donnell School of Public Health, University of Texas Southwestern Medical Center, Dallas, TX 75390 USA.
Laura A BanaszynskiHarold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390 USA.ORCID 0000-0002-5189-8848 Noelle WilliamsDepartment of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390 USA.ORCID 0000-0003-1894-5742 David G McFaddenDepartment of Internal Medicine, Division of Endocrinology, University of Texas Southwestern Medical Center, Dallas, TX 75390 USA.ORCID 0000-0001-8466-2199 Funding
Targeting EWSR1-FLI1 through Functional, Structural and Chemical ApproachesU54CA231649 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI MCKNIGHT, STEVEN L · 2019 to 2019
$10.0MChromatin Dynamics and Genome RegulationR35GM124958 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI Laura Banaszynski · 2017 to 2026
$4.4MMechanisms of p300 Activation During Pluripotency and Differentiation.R01HD109239 · NICHD · UT SOUTHWESTERN MEDICAL CENTER · PI Laura Banaszynski · 2022 to 2026
$2.1MNCI NIH HHS U54 CA231649NICHD NIH HHS R01 HD109239NIGMS NIH HHS R35 GM124958
6 · The paper itselfAbstract
Pediatric malignancies frequently harbor chromosomal translocations that induce expression of fusion oncoproteins. The EWSR1-FLI1 fusion oncoprotein acts as a neomorphic transcription factor and is the dominant genetic driver of Ewing's sarcoma. Interrogation of the mechanisms by which EWSR1-FLI1 drives tumorigenesis has been limited by a lack of model systems to precisely and selectively control its expression in patient-derived cell lines and xenografts. Here, we report the generation of a panel of patient-derived EWS cell lines in which inducible protein degrons were engineered into the endogenous EWSR1-FLI1 locus. These alleles enabled rapid and efficient depletion of EWSR1-FLI1. Complete suppression of EWSR1-FLI1 induced a reversible cell cycle arrest at the G
Indexed as
auxin-inducible degronEwing’s sarcomaEWSR1-FLI1fusion oncoproteininducible degronsmall molecule assisted shutoffxenograft
Identifiers
PMID39554175
PMCPMC11566046
What Socratic holds
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