Evidence map›Paper›PMID 39558621›Full record

ArticleRheumatology (Oxford, England)2025

Cell-integrated serum-induced signalling patterns can differentiate between hand and knee osteoarthritis patients.

Margot Neefjes, Bas A C Housmans, Charlotte Kaffa, Nathalie G M Thielen, Leo A B Joosten, Cornelia H M van den Ende, Elly L Vitters, Guus G H van den Akker, Tim J M Welting, Arjan P M van Caam and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Rheumatology (Oxford, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Margot NeefjesDepartment of Rheumatology, Radboud University Medical Center, Nijmegen, The Netherlands.
Bas A C HousmansLaboratory for Experimental Orthopedics, Department of Orthopedic Surgery, Maastricht University, Maastricht, The Netherlands.
Charlotte KaffaCenter for Molecular and Biomolecular Informatics, Radboud University Medical Center, Nijmegen, The Netherlands.
Nathalie G M ThielenDepartment of Rheumatology, Radboud University Medical Center, Nijmegen, The Netherlands.
Leo A B JoostenDepartment of Internal Medicine, Radboud Institute of Molecular Life Sciences (RIMLS), Radboud University Medical Center, Nijmegen, The Netherlands.
Cornelia H M van den EndeDepartment of Rheumatology, Radboud University Medical Center, Nijmegen, The Netherlands.
Elly L VittersDepartment of Rheumatology, Radboud University Medical Center, Nijmegen, The Netherlands.
Guus G H van den AkkerLaboratory for Experimental Orthopedics, Department of Orthopedic Surgery, Maastricht University, Maastricht, The Netherlands.
Tim J M WeltingLaboratory for Experimental Orthopedics, Department of Orthopedic Surgery, Maastricht University, Maastricht, The Netherlands.
Arjan P M van CaamDepartment of Rheumatology, Radboud University Medical Center, Nijmegen, The Netherlands.
Peter M van der KraanDepartment of Rheumatology, Radboud University Medical Center, Nijmegen, The Netherlands.

Funding

NWO #P15-23TTW PerspectiefWilliam Hunter Revisited
6 · The paper itself

Abstract

objectiveOA is a very heterogeneous disease. Here, we aimed to differentiate OA patients based on their serum-induced cell-integrated signalling patterns.

designIn order to monitor the activity of different cellular homeostasis-regulating pathways in response to patient serum, we analysed the response of human OA serum samples to 16 cell-based transcription factor luciferase reporter assays. In this study we compared serum samples from 55 patients with knee OA, 56 patients with hand OA and 42 healthy controls.

resultsDifferential serum-induced pathway activity was observed between samples from healthy controls, knee OA and hand OA patients: serum of hand OA patients induced high MAPK-related AP1 activity whereas serum of knee OA patients induced more SRE, ISRE and SOX9 activity, which is related to ELK1-SRF, STAT1-STAT2 and SOX9 activity, respectively. Principal component analysis revealed that these differences differentiate hand OA from knee OA. Both hand and knee OA clustered clearly in two different endotypes each, but no principle component could be identified of these subtypes within either the hand OA or the knee OA sample group.

conclusionThis study demonstrates that serum from hand OA and knee OA patients evokes diverse cellular signalling patterns that differentiates hand OA, knee OA and healthy controls. This underlines that the pathomolecular mechanisms of OA are likely significantly different between hand and knee OA, a finding that could lead to new insight into the pathobiology of OA endotypes and joint-specific therapies.

Indexed as

Hand JointsOsteoarthritisOsteoarthritis, KneeSignal TransductionAdultAgedCase-Control StudiesDiagnosis, DifferentialFemaleHumansMaleMiddle AgedSOX9 Transcription FactorSOX9 Transcription Factorbioassayendotypeosteoarthritisserumstratification

Identifiers

PMID39558621
PMCPMC12107062

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.