Evidence map›Paper›PMID 39578398›Full record

ArticleBiotechnology and bioengineering2025

Expression of Viral DNA Polymerase in Synthetic Recombinant Adeno-Associated Virus Producer Cell Line Enhances Full Particle Productivity.

Yu-Chieh Lin, Han-Jung Kuo, Min Lu, Carissa Rungkittikhun, Wei-Shou Hu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology and bioengineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yu-Chieh LinDepartment of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, Minnesota, USA.
Han-Jung KuoDepartment of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, Minnesota, USA.
Min LuDepartment of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, Minnesota, USA.
Carissa RungkittikhunDepartment of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, Minnesota, USA.
Wei-Shou HuDepartment of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, Minnesota, USA.ORCID 0000-0001-7739-8692

Funding

The authors received no specific funding for this work.
6 · The paper itself

Abstract

Recombinant adeno-associated virus (rAAV) is a widely used viral vector in gene therapy. To meet the growing clinical demand, a scalable production technology which can efficiently produce high-quality products is required. We have developed a synthetic biology strategy to generate HEK293-based cell lines which have integrated essential AAV and adenoviral helper genes and are capable of producing rAAV upon induction. One such cell line, GX6B, produced up to 10

Indexed as

DependovirusDNA-Directed DNA PolymeraseCell LineGenetic VectorsHEK293 CellsHerpesvirus 1, HumanHumansViral ProteinsVirionDNA-Directed DNA PolymeraseViral ProteinsDNA polymerasegene therapyHerpes Simplex Virusrecombinant adeno‐associated virussynthetic biologyviral vector manufacturing technology

Identifiers

PMID39578398
PMCPMC11718424

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.