Evidence map›Paper›PMID 39578791›Full record

ArticleReproductive biology and endocrinology : RB&E2024

Dendritic cells under the control of the preimplantation embryo secretome: an in vitro study.

Christiana Kyvelidou, Sofia Haselrieder, Maria von Gierke, Johanna M Gostner, Wolfgang Biasio, Barbara Wirleitner, Christine Heufler, Bettina Toth, Susanne Hofer-Tollinger

Abstract readMulticenter Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproductive biology and endocrinology : RB&E, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Christiana KyvelidouDepartment of Gynaecological Endocrinology and Reproductive Medicine, Medical University of Innsbruck, Anichstraße 35, Innsbruck, 6020, Austria.
Sofia HaselriederDepartment of Gynaecological Endocrinology and Reproductive Medicine, Medical University of Innsbruck, Anichstraße 35, Innsbruck, 6020, Austria.
Maria von GierkeDepartment of Gynaecological Endocrinology and Reproductive Medicine, Medical University of Innsbruck, Anichstraße 35, Innsbruck, 6020, Austria.
Johanna M GostnerInstitute of Medical Biochemistry, Biochemical Immunotoxicology Group, Biocenter, Medical University of Innsbruck, Innsbruck, Austria.
Wolfgang BiasioDepartment of Gynaecological Endocrinology and Reproductive Medicine, Medical University of Innsbruck, Anichstraße 35, Innsbruck, 6020, Austria.
Barbara WirleitnerNext Fertility IVF Prof. Zech, Bregenz GmbH, Bregenz, Austria.
Christine HeuflerDepartment of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria.
Bettina TothDepartment of Gynaecological Endocrinology and Reproductive Medicine, Medical University of Innsbruck, Anichstraße 35, Innsbruck, 6020, Austria.
Susanne Hofer-TollingerDepartment of Gynaecological Endocrinology and Reproductive Medicine, Medical University of Innsbruck, Anichstraße 35, Innsbruck, 6020, Austria. susanne.tollinger@i-med.ac.at.

Funding

Austrian Science Fund P33249-B
6 · The paper itself

Abstract

objectiveTo study the crosstalk between maternal immune cells and the developing embryo by investigating the immunogenic properties of human blastocyst spent media (SM) on dendritic cells.

methodsIn this prospective multicenter experimental study, human preimplantation embryo spent media were collected after blastocyst formation, grouped based on successful or unsuccessful implantation, and analyzed by protein array or used to stimulate monocyte derived dendritic cells (moDC). The immunomodulatory properties of SM on moDC were investigated by analyzing changes in phenotype, cytokine secretion, indoleamine 2,3-dioxygenase (IDO) activity, and ability to activate T cells.

resultsA plethora of cytokines and growth factors secreted from preimplantation embryos was detected. Exposure to embryo SM altered the phenotype of moDC in a manner dependent on the implantation outcome. Specifically, SM from non-implanted embryos increased the expression of co-stimulatory molecules and activation markers on moDC. Furthermore, SM treated dendritic cells secreted low levels of cytokines and growth factors and were able to stimulate naïve T cells. Activation of IDO was decreased in moDC after stimulation with SM.

conclusionsOur findings show that human preimplantation embryos secrete an abundance of molecules with the ability to significantly affect and even regulate immune cells in their environment.

Indexed as

BlastocystCytokinesDendritic CellsCells, CulturedCulture MediaEmbryo Culture TechniquesEmbryo ImplantationEmbryonic DevelopmentFemaleHumansIndoleamine-Pyrrole 2,3,-DioxygenasePregnancyProspective StudiesT-LymphocytesCulture MediaCytokinesIndoleamine-Pyrrole 2,3,-DioxygenaseBlastocystDendritic cellsEmbryo spent mediaMaternal tolerancePreimplantation embryo

Identifiers

PMID39578791
PMCPMC11585248

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.