Evidence map›Paper›PMID 39581338›Full record

ArticleAnalytical biochemistry2025

Studying C9orf72 dipeptide repeat polypeptide aggregation using an analytical ultracentrifuge equipped with fluorescence detection.

Bashkim Kokona, Nicole R Cunningham, Jeanne M Quinn, Danielle R Jacobsen, F Jay Garcia, Sierra M Galindo, Leonard Petrucelli, Walter F Stafford, Thomas M Laue, Robert Fairman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Bashkim KokonaDepartment of Biology, Haverford College, 370 Lancaster Ave, Haverford, PA, 19041, USA.
Nicole R CunninghamDepartment of Biology, Haverford College, 370 Lancaster Ave, Haverford, PA, 19041, USA.
Jeanne M QuinnDepartment of Biology, Haverford College, 370 Lancaster Ave, Haverford, PA, 19041, USA.
Danielle R JacobsenDepartment of Biology, Haverford College, 370 Lancaster Ave, Haverford, PA, 19041, USA.
F Jay GarciaDepartment of Biology, Haverford College, 370 Lancaster Ave, Haverford, PA, 19041, USA.
Sierra M GalindoDepartment of Biology, Haverford College, 370 Lancaster Ave, Haverford, PA, 19041, USA.
Leonard PetrucelliDepartment of Neuroscience, Mayo Clinic, 4500 San Pablo Road, Jacksonville, FL, 32224, USA; Neurobiology of Disease Graduate Program, Mayo Graduate School, Mayo Clinic College of Medicine, Rochester, MN, 55905, USA.
Walter F StaffordBoston Biomedical Research Institute, Watertown, MA, 02472, USA.
Thomas M LaueDepartment of Molecular, Cellular & Biomedical Sciences, University of New Hampshire, 46 College Road, Durham, NH, 03824, USA.
Robert FairmanDepartment of Biology, Haverford College, 370 Lancaster Ave, Haverford, PA, 19041, USA. Electronic address: rfairman@haverford.edu.

Funding

Resource Component: Acquisition, maintenance and distribution of Drosophila stocksP40OD018537 · OD · TRUSTEES OF INDIANA UNIVERSITY · PI Annette L. Parks · 2014 to 2026
$13.5M
In vivo and crude extract analysis of polyQ aggregation intermediatesR15NS081681 · NINDS · HAVERFORD COLLEGE · PI FAIRMAN, ROBERT · 2012 to 2012
$351k
NIH HHS P40 OD018537NINDS NIH HHS R15 NS081681
6 · The paper itself

Abstract

Sedimentation velocity, using an analytical ultracentrifuge equipped with fluorescence detection, and electrophoresis methods are used to study aggregation of proteins in transgenic animal model systems. Our previous work validated the power of this approach in an analysis of mutant huntingtin aggregation. We demonstrate that this method can be applied to another neurodegenerative disease studying the aggregation of three dipeptide repeats (DPRs) produced by aberrant translation of mutant c9orf72 containing large G

Indexed as

C9orf72 ProteinCaenorhabditis elegansDipeptidesAmyotrophic Lateral SclerosisAnimalsDrosophila melanogasterFluorescenceFrontotemporal DementiaHumansPeptidesProtein AggregatesUltracentrifugationC9orf72 ProteinC9orf72 protein, humanDipeptidesPeptidesProtein AggregatesAmyotrophic lateral sclerosisAnalytical ultracentrifugationDrosophila melanogasterSedimentation velocitySemi-denaturing detergent agarose gel electrophoresis

Identifiers

PMID39581338
PMCPMC11624972

What Socratic holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.