Evidence map›Paper›PMID 39582686›Full record

ReviewJournal of extracellular biology2024

Uromodulin and the study of urinary extracellular vesicles.

Michael A Harding, Hayrettin Yavuz, Annika Gathmann, Samantha Upson, Agnieszka Swiatecka-Urban, Uta Erdbrügger

Erratum issuedAbstract readReview
In one paragraph

Review in Journal of extracellular biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Uromodulin and the study of urinary extracellular vesicles.Journal of extracellular biology · 2024
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Michael A HardingDivision of Nephrology, Department of Medicine University of Virginia Charlottesville Virginia USA.
Hayrettin YavuzDivision of Pediatric Nephrology, Department of Medicine University of Virginia Charlottesville Virginia USA.ORCID https://orcid.org/0000-0001-5190-7022
Annika GathmannSchool of Medicine University of Hamburg Hamburg Germany.
Samantha UpsonDivision of Nephrology, Department of Medicine University of Virginia Charlottesville Virginia USA.
Agnieszka Swiatecka-UrbanDivision of Pediatric Nephrology, Department of Medicine University of Virginia Charlottesville Virginia USA.
Uta ErdbrüggerDivision of Nephrology, Department of Medicine University of Virginia Charlottesville Virginia USA.

Funding

Correcting Pathogenic TGF beta Activity in the AirwayR01HL144539 · NHLBI · UNIVERSITY OF VIRGINIA · PI SWIATECKA-URBAN, AGNIESZKA · 2019 to 2022
$1.7M
NHLBI NIH HHS R01 HL144539
6 · The paper itself

Abstract

Urinary extracellular vesicles (uEVs) are a promising substrate for discovering new biomarkers. In order to investigate the origin of uEVs and the cargo they carry, some types of downstream analysis of uEVs may require concentration and enrichment as well as removal of contaminating substances. Co-isolation of the abundant urinary protein uromodulin with uEVs can be a problem, and may interfere with some techniques, in particular with proteomic analysis tools. Methods of separating out uromodulin and its removal have also not been standardized. This review highlights aspects of uromodulin structure that makes it recalcitrant to separation from uEVs, summarizes frequently used techniques for uEV enrichment and how they affect uromodulin separation, and specific methods for uromodulin removal during preparation of uEVs. The necessity of uromodulin removal for various study endpoints is also examined.

Indexed as

extracellular vesiclesTamm–Horsfall proteinuEVsurineuromodulin

Identifiers

PMID39582686
PMCPMC11583080

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.