ArticleScientific reports2024
Metabolomics analysis reveals resembling metabolites between humanized γδ TCR mice and human plasma.
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Targeted replacement of human γδ TCR in mice enhances antigen-specific B cell immunity.Frontiers in immunology · 2026Article
- Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gamma delta (γδ) T cells, which reside in mucosal and epithelial tissues, are integral to immune responses and are involved in various cancers, autoimmune, and infectious diseases. To study human γδ T cells to a translational level, we developed γδ humanized TCR-T1 (HuTCR-T1) mice using our TruHumanization platform. We compared the metabolomic profiles from plasma samples of wild-type (WT), γδ HuTCR-T1 mice, and humans using UHPLC-MS/MS. Untargeted metabolomics and lipidomics were used to screen all detectable metabolites. Principal component analysis revealed that the metabolomic profiles of γδ HuTCR-T1 mice closely resemble those of humans, with a clear segregation of metabolites between γδ HuTCR-T1 and WT mice. Most humanized γδ metabolites were classified as lipids, followed by organic compounds and amino acids. Pathway analysis identified significant alterations in the metabolism of tryptophan, tyrosine, sphingolipids, and glycerophospholipids, shifting these pathways towards a more human-like profile. Immunophenotyping showed that γδ HuTCR-T1 mice maintained normal proportions of both lymphoid and myeloid immune cell populations, closely resembling WT mice, with only a few exceptions. These findings demonstrate that the γδ HuTCR-T1 mouse model exhibits a metabolomic profile that is remarkably similar to that of humans, highlighting its potential as a relevant model for investigating the role of metabolites in disease development and progression. This model also offers an opportunity to discover therapeutic human TCRs.
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Registered trials
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