DLK1 Is Associated with Stemness Phenotype in Medullary Thyroid Carcinoma Cell Lines.

Danilo Dias da Silva, Rodrigo Pinheiro Araldi, Mariana Rocha Belizario, Welbert Gomes Rocha, Rui Monteiro de Barros Maciel, Janete Maria Cerutti

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Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

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2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Danilo Dias da SilvaGenetic Bases of Thyroid Tumour Laboratory, Division of Genetics, Department of Morphology and Genetics, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04039-032, SP, Brazil.

Rodrigo Pinheiro AraldiGenetic Bases of Thyroid Tumour Laboratory, Division of Genetics, Department of Morphology and Genetics, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04039-032, SP, Brazil.ORCID 0000-0001-8288-2577

Mariana Rocha BelizarioGenetic Bases of Thyroid Tumour Laboratory, Division of Genetics, Department of Morphology and Genetics, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04039-032, SP, Brazil.ORCID 0009-0000-5518-2647

Welbert Gomes RochaGenetic Bases of Thyroid Tumour Laboratory, Division of Genetics, Department of Morphology and Genetics, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04039-032, SP, Brazil.

Rui Monteiro de Barros MacielLaboratório de Endocrinologia Molecular e Translacional, Disciplina de Endocrinologia e Metabologia, Departamento de Medicina, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04039-032, SP, Brazil.ORCID 0000-0002-6123-6098

Janete Maria CeruttiGenetic Bases of Thyroid Tumour Laboratory, Division of Genetics, Department of Morphology and Genetics, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04039-032, SP, Brazil.ORCID 0000-0003-0156-8274

Funding

CAPES 88882.430339/2019-01CNPq 305690/2022-1 and 308338/2020-0FAPEPSP research grants (2022/09713-9 and 2021/02752-6) and scholarship (2023/16471-4 and 2022/16827-0).

6 · The paper itself

Abstract

Medullary thyroid carcinoma (MTC) is a rare and aggressive tumor, often requiring systemic treatment in advanced or metastatic stages, where drug resistance presents a significant challenge. Given the role of cancer stem cells (CSCs) in cancer recurrence and drug resistance, we aimed to identify CSC subpopulations within two MTC cell lines harboring pathogenic variants in the two most common MEN2-associated codons. We analyzed 15 stemness-associated markers, along with well-established thyroid stem cell markers (CD133, CD44, and ALDH1), a novel candidate (DLK1), and multidrug resistance proteins (MRP1 and MRP3). The ability to efflux the fluorescent dye Hoechst 3342 and form spheroids, representing CSC behavior, was also assessed. MZ-CRC-1 cells (p.M918T) displayed higher expressions of canonical markers, DLK1, and MRP proteins than TT cells (p.C634W). MZ-CRC-1 cells also formed more spheroids and showed less dye accumulation (

Indexed as

Calcium-Binding ProteinsCarcinoma, NeuroendocrineNeoplastic Stem CellsThyroid NeoplasmsBiomarkers, TumorCell Line, TumorHumansIntercellular Signaling Peptides and ProteinsMembrane ProteinsPhenotypeSpheroids, CellularBiomarkers, TumorCalcium-Binding ProteinsDLK1 protein, humanIntercellular Signaling Peptides and ProteinsMembrane Proteinscancer stem cellsCD133CD44DLK1medullary thyroid carcinomap.M918TRET

Identifiers

PMID39595993

DOI10.3390/ijms252211924

PMCPMC11594232

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Textmetadata

LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.