Evidence map›Paper›PMID 39605614›Full record

ArticlebioRxiv : the preprint server for biology2024

Sequence specificity of an essential nuclear localization sequence in Mcm3.

Ziyi Wang, Yun Jing Zhang, Qian-Yi Zhang, Kate Bilsborrow, Matthew Leslie, Raymond T Suhandynata, Huilin Zhou

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Ziyi WangBiomedical Science graduate program, School of Medicine, University of California at San Diego.
Yun Jing ZhangDepartment of Cellular and Molecular Medicine, School of Medicine, University of California at San Diego.ORCID 0009-0001-6261-8661
Qian-Yi ZhangBiomedical Science graduate program, School of Medicine, University of California at San Diego.
Kate BilsborrowDepartment of Cellular and Molecular Medicine, School of Medicine, University of California at San Diego.
Matthew LeslieDepartment of Cellular and Molecular Medicine, School of Medicine, University of California at San Diego.
Raymond T SuhandynataDepartment of Pathology, School of Medicine, University of California at San Diego.
Huilin ZhouBiomedical Science graduate program, School of Medicine, University of California at San Diego.ORCID 0000-0002-1350-4430

Funding

Suppression of duplication-mediated genome rearrangements by protein sumoylationR01GM116897 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ZHOU, HUILIN · 2015 to 2023
$2.6M
Kinetochore Assembly and RegulationR01GM151191 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Arshad Desai, Raymond Theodore Suhandynata · 2023 to 2026
$1.8M
NIGMS NIH HHS R01 GM116897NIGMS NIH HHS R01 GM151191
6 · The paper itself

Abstract

Proteins with nuclear localization sequences (NLSs) are directed into the cell nucleus through interactions between the NLS and importin proteins. NLSs are generally short motifs rich in basic amino acids; however, identifying NLSs can be challenging due to the lack of a universally conserved sequence. In this study, we characterized the sequence specificity of an essential and conserved NLS in Mcm3, a subunit of the replicative DNA helicase. Through mutagenesis and AlphaFold 3 (AF3) modeling, we demonstrate that the precise positioning of basic residues within the NLS is critical for nuclear transport of Mcm3 through optimal interactions with importin. Disrupting these interactions impairs the nuclear import of Mcm3, resulting in defective chromatin loading of MCM and poor cell growth. Our results provide a structure-guided framework for predicting and analyzing monopartite NLSs, which, despite lacking a single consensus sequence, retain key characteristics shared between the NLSs of Mcm3 and the SV40 large T antigen.

Indexed as

AlphaFold 3importin alphaMCM loadingNuclear localization sequence (NLS)

Identifiers

PMID39605614
PMCPMC11601334

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.