Evidence map›Paper›PMID 39617900›Full record

ArticleMolecular medicine (Cambridge, Mass.)2024

Ginsenoside Rb1 affects mitochondrial Ca

Yuesong Yin, Hai Hu, Yian Yang, Song Wu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine (Cambridge, Mass.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Article
  5. Review
  6. Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yuesong YinOrthopedics Department, The Third Xiangya Hospital of Central South University, No.138 Tongzipo Road, Yuelu District, Changsha, 410013, China. 2204160408@csu.edu.cn.
Hai HuOrthopedics Department, The Third Xiangya Hospital of Central South University, No.138 Tongzipo Road, Yuelu District, Changsha, 410013, China.
Yian YangOncology Department, The Third Xiangya Hospital of Central South University, Changsha, 410013, China.
Song WuOrthopedics Department, The Third Xiangya Hospital of Central South University, No.138 Tongzipo Road, Yuelu District, Changsha, 410013, China. xy3ws1969@hotmail.com.

Funding

National Natural Science Foundation of China 82072501Science and Technology Innovation Leading Plan of High Tech Industry in Hunan Province 2020SK2011
6 · The paper itself

Abstract

backgroundRotator cuff tears (RCTs) are among the most common musculoskeletal disorders that affect quality of life. This study aimed to investigate the efficacy of ginsenoside Rb1 in RCTs and the mechanisms involved.

methodsFirst, a fibrotic model of FAPs was induced, and FAPs were cultured in media supplemented with different concentrations of ginsenoside Rb1. Next, a rat model of RCTs was constructed and treated with ginsenoside Rb1. Molecular docking was subsequently utilized to detect the binding of ginsenoside Rb1 and SFRP1. Finally, SFRP1 was knocked down and overexpressed in vivo and in vitro to investigate the mechanism of ginsenoside Rb1 and SFRP1 in RCTs.

resultsCompared with the Normal group, FAP viability was decreased, but Collagen II, FN and α-SMA levels were increased in the Control group. After treatment with different concentrations of ginsenoside Rb1, FAP viability increased, but Collagen II, FN and α-SMA levels decreased. Among them, 60 µM ginsenoside Rb1 had the best effect. In vivo experiments revealed that ginsenoside Rb1 improved RCTs in rats. Molecular docking revealed the binding of ginsenoside Rb1 to SFRP1. Additionally, SFRP1 levels were lower in the Control group than in the Normal group. After treatment with ginsenoside Rb1, SFRP1 levels increased. In vivo, overexpressing SFRP1 along with ginsenoside Rb1 treatment further alleviated tendon tissue fibroblast infiltration and fat accumulation and further reduced the expression of Collagen II, FN, and α-SMA. In vitro, overexpressing SFRP1 along with ginsenoside Rb1 treatment further decreased the expression of CaMKII, PLC, PKC, Wnt, and β-catenin, further decreased the Ca

conclusionsGinsenoside Rb1 inhibited the activation of the Wnt signaling pathway by promoting SFRP1 expression, thereby inhibiting mitochondrial function and Ca

Indexed as

CalciumFibrosisGinsenosidesMitochondriaMolecular Docking SimulationRotator Cuff InjuriesWnt Signaling PathwayAnimalsDisease Models, AnimalIntercellular Signaling Peptides and ProteinsMaleMembrane ProteinsRatsRats, Sprague-DawleySecreted Frizzled-Related ProteinsCalciumginsenoside Rb1GinsenosidesIntercellular Signaling Peptides and ProteinsMembrane ProteinsSecreted Frizzled-Related ProteinsSfrp1 protein, ratCa2+Ginsenoside Rb1Rotator cuff tearsSFRP1Wnt

Identifiers

PMID39617900
PMCPMC11610219

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.