Assembling and dietary application of a local trnL metabarcoding database for

Yuqin Liu, Dandan Wang, Zhiming Cao, Wuhua Liu, Zechun Bao, Weiwei Zhang, Yongtao Xu

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In one paragraph

Article in Biodiversity data journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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5 · Who and what money

Authors and funding

7 authors.

Yuqin LiuJiangxi Provincial Key Laboratory for Conservation Biology, Jiangxi Agricultural University, Nanchang, China Jiangxi Provincial Key Laboratory for Conservation Biology, Jiangxi Agricultural University Nanchang China.

Dandan WangJiangxi Provincial Key Laboratory for Conservation Biology, Jiangxi Agricultural University, Nanchang, China Jiangxi Provincial Key Laboratory for Conservation Biology, Jiangxi Agricultural University Nanchang China.

Zhiming CaoJiangxi Provincial Key Laboratory for Conservation Biology, Jiangxi Agricultural University, Nanchang, China Jiangxi Provincial Key Laboratory for Conservation Biology, Jiangxi Agricultural University Nanchang China.

Wuhua LiuTaohongling Sika Deer National Nature Reserve Administration, Pengze, China Taohongling Sika Deer National Nature Reserve Administration Pengze China.

Zechun BaoJiangxi Lushan National Nature Reserve Administration, Jiujiang, China Jiangxi Lushan National Nature Reserve Administration Jiujiang China.

Weiwei ZhangJiangxi Provincial Key Laboratory for Conservation Biology, Jiangxi Agricultural University, Nanchang, China Jiangxi Provincial Key Laboratory for Conservation Biology, Jiangxi Agricultural University Nanchang China.

Yongtao XuJiangxi Provincial Key Laboratory for Conservation Biology, Jiangxi Agricultural University, Nanchang, China Jiangxi Provincial Key Laboratory for Conservation Biology, Jiangxi Agricultural University Nanchang China.ORCID https://orcid.org/0000-0002-0159-2031

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The quality and completeness of the reference database have a direct impact on the accuracy of forage plant identification, thereby influencing the level of conservation and management of wildlife resources. In our research, target amplification was subjected to first-generation sequencing to assemble a local reference database using chloroplast trnL metabarcoding. We found that the primers c-h outperformed g-h as a universal DNA metabarcoding and 162 valid choloroplast trnL sequences were submitted (GenBank ID: PP081756 - PP081917), which exhibited an obvious preference for A and T nucleotides (60.49%). The haplotype diversity (

Indexed as

Cervusnipponkopschidiethigh-throughput sequencinglocal databasetrnL metabarcoding

Identifiers

PMID39619293

DOI10.3897/BDJ.12.e139269

PMCPMC11605298

What Socratic holds

Textmetadata

LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.