Evidence map›Paper›PMID 39620591›Full record

ArticleJournal of visualized experiments : JoVE2024

Novel Quantification Protocol for Cardiovascular Calcification Progression Using Longitudinal MicroPET/MicroCT Images.

Isabel L Day, Mikayla Tamboline, Andrea Litwak, Andrea Sarabia, Yin Tintut, Linda L Demer, Shili Xu

Abstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of visualized experiments : JoVE, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Isabel L DayDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles; Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of California, Los Angeles.
Mikayla TambolineDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles; Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of California, Los Angeles.
Andrea LitwakDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles; Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of California, Los Angeles.
Andrea SarabiaDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles; Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of California, Los Angeles.
Yin TintutDepartment of Medicine, David Geffen School of Medicine, University of California, Los Angeles; Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles; Department of Orthopaedic Surgery, David Geffen School of Medicine, University of California, Los Angeles.
Linda L DemerDepartment of Medicine, David Geffen School of Medicine, University of California, Los Angeles; Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles; Department of Bioengineering, University of California, Los Angeles.
Shili XuDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles; Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of California, Los Angeles; Jonsson Comprehensive Cancer Center, University of California, Los Angeles; ShiliXu@mednet.ucla.edu.

Funding

Tumor Immunology and Immunotherapy (TII)P30CA016042 · UNIVERSITY OF CALIFORNIA LOS ANGELES · 1985 to 2025
$29.3M
Role of intermittent activation of parathyroid hormone receptor in exercise-induced vascular calcificationR01HL151391 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Linda L. Demer, Yin Tintut · 2022 to 2024
$1.6M
NCI NIH HHS P30 CA016042NHLBI NIH HHS R01 HL137647NHLBI NIH HHS R01 HL151391NIH HHS S10 OD026917
6 · The paper itself

Abstract

Micro positron emission tomography (PET) and micro computed tomography (CT) imaging are powerful, ideal research tools for following the progression of cardiovascular calcification. Due to their non-invasive nature, small research animals can be imaged at multiple time points. The challenge lies in the accurate quantification of cardiovascular calcification. Here, we provide a protocol, using images from the later disease stages as a template, to accurately quantify the progression of cardiovascular calcification in longitudinal studies. The protocol involves 1) the alignment of the chest area in multiple images from the same animal during a longitudinal study as the first step, 2) the definition of a region of interest (ROI) situated within the heart and the aorta at the site of larger calcium deposits that become apparent in later images, and 3) simultaneous segmentation and quantification of calcium deposits across all images acquired during the longitudinal study. This streamlined method enhances the accuracy of image analysis in following the progression of cardiovascular calcification by improving the precision of ROI definition and reducing the variability associated with earlier techniques that analyze individual scans independently.

Indexed as

X-Ray MicrotomographyAnimalsCalcinosisDisease ProgressionMicePositron-Emission TomographyVascular Calcification

Identifiers

PMID39620591
PMCPMC12108129

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.