Evidence mapPaperPMID 39624136Full record

ArticlePeerJ2024

PSENEN influences the progression of renal clear cell carcinoma by regulating the immune microenvironment and oxidative phosphorylation.

Congying Huang, Kaijie Chen, Siyu Zhu, Xin Yang, Jiangang Hou, Xuefeng Gu

Abstract read
In one paragraph

Article in PeerJ, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Congying Huang *School of Pharmacy, Shanghai University of Medicine & Health Sciences, Shanghai, Shanghai, China.
Kaijie Chen *School of Pharmacy, Shanghai University of Medicine & Health Sciences, Shanghai, Shanghai, China.
Siyu ZhuSchool of Pharmacy, Shanghai University of Medicine & Health Sciences, Shanghai, Shanghai, China.
Xin YangDepartment of Endocrinology, Putuo People's Hospital, Tongji University, Shanghai, Shanghai, China.
Jiangang HouDepartment of Urology, Huashan Hospital, Fudan University, Shanghai, Shanghai, China.
Xuefeng GuSchool of Pharmacy, Shanghai University of Medicine & Health Sciences, Shanghai, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Presenilin enhancer gamma-secretase subunit (PSENEN), the straight target of metformin, is highly expressed in several cancers. The role of PSENEN in kidney renal clear cell carcinoma (KIRC) has not been reported. Methods: PSENEN expression in KIRC specimens was investigated in The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, as well as by immunohistochemical analysis and qPCR assay. The relationship between PSENEN expression and patient survival was discussed. The biological function of PSENEN in KIRC and its correlation with immune infiltration of KIRC were then investigated, and possible cellular mechanisms were again analyzed. The effects of metformin on KIRC cell proliferation, migration and invasion were discussed in cellular experiments. Results: PSENEN was found to be highly expressed in KIRC. The high PSENEN expression was an adverse factor in KIRC. Several immune-related pathways were enriched including immune response, complement and coagulation cascade reactions, and neutrophil extracellular trap formation, as evidenced by enrichment analyses. Immune infiltration analysis revealed that PSENEN expression correlated positively with regulatory T cells. Gene set variation analysis suggested that PSENEN expression correlated positively with oxidative phosphorylation. In addition, a certain concentration of metformin was found to inhibit the proliferation, migration and invasion of KIRC cells, in which PSENEN down-regulation, AMPK up-regulation and mTOR down-regulation were also observed. Conclusions: PSENEN may be involved in regulating the immune microenvironment of KIRC, and oxidative phosphorylation may also be a pathway for its involvement in cancer development. PSENEN is a novel prognostic marker for KIRC.

Indexed as

Carcinoma, Renal CellCell ProliferationDisease ProgressionKidney NeoplasmsOxidative PhosphorylationTumor MicroenvironmentCell Line, TumorCell MovementFemaleGene Expression Regulation, NeoplasticHumansMaleMetforminMiddle AgedMetforminImmune microenvironmentKidney renal clear cell carcinomaMetforminOxidative phosphorylationPrognosisPSENEN (PEN2)

Identifiers

PMID39624136
PMCPMC11610461

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.