Evidence map›Paper›PMID 39627821›Full record

ArticleStem cell research & therapy2024

Dynamic three dimensional environment for efficient and large scale generation of smooth muscle cells from hiPSCs.

Akazha Green, Yuhua Wei, Jason M Warram, Yolanda E Hartman, Xiaoxiao Geng, Thanh Nguyen, Lei Ye, Jianyi Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Akazha GreenDepartment of Biomedical Engineering, The University of Alabama at Birmingham, Volker Hall, 1670 University Boulevard, Birmingham, AL, 35255, USA.
Yuhua WeiDepartment of Biomedical Engineering, The University of Alabama at Birmingham, Volker Hall, 1670 University Boulevard, Birmingham, AL, 35255, USA.
Jason M WarramDepartment of Biomedical Engineering, The University of Alabama at Birmingham, Volker Hall, 1670 University Boulevard, Birmingham, AL, 35255, USA.
Yolanda E HartmanDepartment of Biomedical Engineering, The University of Alabama at Birmingham, Volker Hall, 1670 University Boulevard, Birmingham, AL, 35255, USA.
Xiaoxiao GengDepartment of Biomedical Engineering, The University of Alabama at Birmingham, Volker Hall, 1670 University Boulevard, Birmingham, AL, 35255, USA.
Thanh NguyenDepartment of Biomedical Engineering, The University of Alabama at Birmingham, Volker Hall, 1670 University Boulevard, Birmingham, AL, 35255, USA.
Lei YeDepartment of Biomedical Engineering, The University of Alabama at Birmingham, Volker Hall, 1670 University Boulevard, Birmingham, AL, 35255, USA. lye@uab.edu.
Jianyi ZhangDepartment of Biomedical Engineering, The University of Alabama at Birmingham, Volker Hall, 1670 University Boulevard, Birmingham, AL, 35255, USA. jayzhang@uab.edu.

Funding

Project 3 - Role of Proline Metabolism in Regulation of Mammalian Cardiomyocyte ProliferationP01HL160476 · NHLBI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI Hesham Sadek · 2022 to 2026
$13.1M
Integrated Cellular and Tissue Engineering for Ischemic Heart DiseaseU01HL134764 · NHLBI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI BURSAC, NENAD, KAMP, TIMOTHY J. · 2016 to 2022
$7.7M
Supplement of HL131017: Myocardial remuscularization by cardiac patch delivery of epicardial FSTL1 and CCND2 overexpressing cardiomyocytesR01HL131017 · NHLBI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI SERPOOSHAN, VAHID, ZHANG, JIANYI · 2016 to 2025
$5.8M
Development and Functional Assessment of Cardiovascular Tissue Engineering Therapy (CVTE) T32 Training GrantT32EB023872 · NIBIB · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI ROGERS, JACK M, ZHANG, JIANYI · 2018 to 2022
$753k
NHLBI NIH HHS P01 HL160476NHLBI NIH HHS P01 HL160476)NHLBI NIH HHS (R01HL11412ONHLBI NIH HHS R01 HL131017NHLBI NIH HHS R01HL131017NHLBI NIH HHS U01 HL134764NHLBI NIH HHS U01HL134764NIBIB NIH HHS T323B023872
6 · The paper itself

Abstract

backgroundChronic ischemic limb disease often leads to amputation, which remains a significant clinical problem. Smooth-muscle cells (SMCs) are crucially involved in the development and progression of many cardiovascular diseases, but studies with primary human SMCs have been limited by a lack of availability. Here, we evaluated the efficiency of two novel protocols for differentiating human induced-pluripotent stem cells (hiPSCs) into SMCs and assessed their potency for the treatment of ischemic limb disease.

methodshiPSCs were differentiated into SMCs via a conventional two-dimensional (2D) protocol that was conducted entirely with cell monolayers, or via two protocols that consisted of an initial five-day three-dimensional (3D) spheroid phase followed by a six-day 2D monolayer phase (3D + 2D differentiation). The 3D phases were conducted in shaker flasks on an orbital shaker (the 3D + 2D shaker protocol) or in a PBS bioreactor (the 3D + 2D bioreactor protocol). Differentiation efficiency was evaluated via the expression of SMC markers (smooth-muscle actin [SMA], smooth muscle protein 22 [SM22], and Calponin-1), and the biological activity of the differentiated hiPSC-SMCs was evaluated via in-vitro assessments of migration (scratch assay), contraction in response to the treatment with a prostaglandin H2 analog (U46619), and tube formation on Geltrex, as well as in-vivo measurements of perfusion (fluorescence angiography) and vessel density in the limbs of mice that were treated with hiPSC-SMCs after experimentally induced hind-limb ischemia (HLI).

resultsBoth 3D + 2D protocols yielded > 5.6 × 10

conclusionsOur dynamic 3D + 2D protocols produced an exceptionally high yield of hiPSC-SMCs. Transplantation of these hiPSC-SMCs results in significantly improved recovery of ischemic limb after ischemic injury in mice.

Indexed as

Cell DifferentiationInduced Pluripotent Stem CellsMyocytes, Smooth MuscleActinsAnimalsCalponinsCell Culture TechniquesHumansIschemiaMiceMicrofilament ProteinsActinsCalponinsMicrofilament ProteinsCell differentiationHuman induced pluripotent stem cellsIschemic limb diseaseSmooth muscle cells

Identifiers

PMID39627821
PMCPMC11616106

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.