Evidence mapPaperPMID 39629744Full record

ArticleCancer medicine2024

FAM3C Regulates Glioma Cell Proliferation, Invasion, Apoptosis, and Epithelial Mesenchymal Transition via the Notch Pathway.

Xiaochao Xia, Zihao Wang, Lvmeng Song, Yinchuan Cheng, Ping Xiong, Shun Li

Abstract read
In one paragraph

Article in Cancer medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Cancer stem cells synthesize proline to attenuate oxidative stress.The Journal of clinical investigation · 2026
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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Xiaochao XiaDepartments of Neurosurgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.ORCID https://orcid.org/0009-0005-0591-2396
Zihao WangDepartments of Neurosurgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.
Lvmeng SongDepartments of Neurosurgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.
Yinchuan ChengDepartments of Neurosurgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.
Ping XiongDepartments of Neurosurgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.
Shun LiDepartments of Neurosurgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.

Funding

Bureau of Science and Technology Nanchong Municipality 19SXHZ0321Sichuan Medical Science and Technology Innovation Research Association YCH-KY-YCZD2024-054the Affiliated Hospital of North Sichuan Medical College of China 2019ZD001the North Sichuan Medical College CBY23-ZDA01
6 · The paper itself

Abstract

backgroundPrevious studies have implicated the involvement of FAM3C in cancerous development and progression. Herein, we aimed to further investigate the oncological mechanism of FAM3C, specifically in glioma.

methodsWe utilized open-source bioinformatics tools and platforms to analyze the transcriptional expression levels, prognosis, and correlation with clinical variables of FAM3C in gliomas, and subsequently, to hypothesize its potential molecular functions and possibly associated signaling pathways. Following this, glioma tissues were obtained from resected specimens of patients to assess the expression of FAM3C and molecular markers related to epithelial-mesenchymal transition (EMT) and Notch signaling pathways. Furthermore, glioma cell lines were subjected to treatments including FAM3C siRNA knockdown, lentiviral overexpression, and Notch signaling pathway blockade, enabling the investigation of molecular functions of FAM3C in vitro, particularly in EMT and Notch signaling pathways, as well as its effects on cancer cell proliferation, cell cycle progression, apoptosis, and invasion, using assays such as MMT cell proliferation assay, transwell migration, and flow cytometry analysis. Finally, a mouse subcutaneous xenograft model was established to explore the integrative function of FAM3C in glioma growth in vivo.

resultsThe expression level of FAM3C correlated with the progression of glioma grade and served as a prognostic indicator for poor patient outcomes. Subsequent experiments conducted on glioma cell lines, tumor tissues, and mouse models reinforced the close association of FAM3C with processes including glioma cell proliferation, cell cycle progression, apoptosis, and invasion. Additionally, it was observed that FAM3C is involved in the regulation of the Notch signaling pathway.

conclusionsFAM3C emerges as a potential candidate for clinical detection and prognostic biomarker application. Its regulatory role in glioma cell proliferation, cell cycle progression, and modulation of epithelial-mesenchymal transition-driven invasion and migration via the Notch signaling pathway implies its potential to unveil novel therapeutic targets for glioma treatment.

Indexed as

ApoptosisCell ProliferationEpithelial-Mesenchymal TransitionGliomaReceptors, NotchSignal TransductionAnimalsBrain NeoplasmsCell Line, TumorCell MovementCytokinesFemaleGene Expression Regulation, NeoplasticHumansMaleMiceCytokinesFAM3C protein, humanNeoplasm ProteinsReceptors, Notchepithelial mesenchymal transitionFAM3CgliomaNotch pathwaytherapeutic targets

Identifiers

PMID39629744
PMCPMC11615680

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.