Evidence map›Paper›PMID 39630306›Full record

ArticlePlanta2024

Silencing CaPIP5K4-1 leads to decreased male fertility in Capsicum annuum L.

Weifu Kong, Panpan Duan, Yuhang Wang, Tao Zhang, Mianzhu Huang, Jingtao Kang, Lina Wang, Bingqiang Wei, Yajun Chang

Abstract read
PubMed Publisher
In one paragraph

Article in Planta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Weifu KongCollege of Horticulture, Gansu Agricultural University, Lanzhou, 730030, China.ORCID http://orcid.org/0009-0004-5003-445X
Panpan DuanVegetable Institute, Gansu Academy of Agricultural Sciences, Lanzhou, 730070, Gansu, China.
Yuhang WangCollege of Horticulture, Gansu Agricultural University, Lanzhou, 730030, China.
Tao ZhangCollege of Horticulture, Gansu Agricultural University, Lanzhou, 730030, China.
Mianzhu HuangCollege of Horticulture, Gansu Agricultural University, Lanzhou, 730030, China.
Jingtao KangCollege of Horticulture, Gansu Agricultural University, Lanzhou, 730030, China.
Lina WangCollege of Horticulture, Gansu Agricultural University, Lanzhou, 730030, China.
Bingqiang WeiCollege of Horticulture, Gansu Agricultural University, Lanzhou, 730030, China. bqwei@gsau.edu.cn.ORCID http://orcid.org/0000-0003-3119-5712
Yajun ChangInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Mem. Sun Yat-Sen, Nanjing, 210014, China. changyj@cnbg.net.ORCID http://orcid.org/0000-0003-3851-9556

Funding

National Natural Science Foundation of China 32360760Science and Technology Major Project (Agriculture) of Gansu Province, China 24ZDNA005
6 · The paper itself

Abstract

MAIN

conclusionPhosphatidylinositol 4-phosphate 5-kinase gene CaPIP5K4-1 is highly expressed in the pepper anthers. Virus-induced gene silencing of CaPIP5K4-1 leads to reduced male fertility in pepper. The phosphatidylinositol 4-phosphate 5-kinase (PIP5K) is a pivotal enzyme in the phosphatidylinositol signaling pathway, and its crucial involvement in both plant development and stress response has been established. Here, we found that the expression of CaPIP5K4-1 in pepper was significantly higher in the fertile flower buds compared to sterile flower buds. Furthermore, its expression was validated in anthers and pollens by qRT-PCR and RNA-ISH assays, respectively. Its GFP fusion protein was mainly located on the plasma membrane. Silencing CaPIP5K4-1 in fertile pepper accessions resulted in wrinkled pollen grain cell walls, decreased pollen germination efficiency, and inhibited pollen tube growth. The transcription levels of multiple genes in the phosphatidylinositol signaling pathway were also assessed. Five phospholipase C (PLC) genes were downregulated in silenced plants. On the contrary, inositol phosphatase SAC and phosphatase and tensin homolog (PTEN) were upregulated. This study reported the role of CaPIP5K4-1 in pepper male fertility and provided insights into the regulatory mechanisms of PI signaling in pepper.

Indexed as

CapsicumGene Expression Regulation, PlantGene SilencingPhosphotransferases (Alcohol Group Acceptor)Plant ProteinsPollenFertilityFlowersPlant InfertilityPollen TubeSignal Transduction1-phosphatidylinositol-4-phosphate 5-kinasePhosphotransferases (Alcohol Group Acceptor)Plant ProteinsInduced gene silencingMale fertilityPepperPhosphatidylinositol 4-phosphate 5-kinasePIP5K4-1Virus

Identifiers

PMID39630306

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.