Evidence map›Paper›PMID 39636729›Full record

ArticleSTAR protocols2024

Protocol for immunofluorescence staining and large-scale analysis to quantify microglial cell morphology at single-cell resolution in mice.

Frida Lind-Holm Mogensen, Corrado Ameli, Alexander Skupin, Alessandro Michelucci

Abstract read
In one paragraph

Article in STAR protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Frida Lind-Holm MogensenNeuro-Immunology Group, Department of Cancer Research, Luxembourg Institute of Health, 6A, Rue Nicolas-Ernest Barblé, L-1210 Luxembourg, Luxembourg; Faculty of Science, Technology and Medicine, University of Luxembourg, 2, avenue de l'Université, L-4365 Esch-sur-Alzette, Luxembourg. Electronic address: frida.lind-holm@lih.lu.
Corrado AmeliIntegrative Cell Signalling Group, Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 7, Avenue des Hauts-Fourneaux, L-4362 Esch-sur-Alzette, Luxembourg. Electronic address: corrado.ameli@gmail.com.
Alexander SkupinIntegrative Cell Signalling Group, Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 7, Avenue des Hauts-Fourneaux, L-4362 Esch-sur-Alzette, Luxembourg; Department of Neurosciences, University of California, San Diego, 9500 Gillman Drive, La Jolla, CA 92093, USA; Integrative Biophysics, Department of Physics and Material Science, University of Luxembourg, 162a, Avenue de la Faïencerie, L-1511 Luxembourg, Luxembourg.
Alessandro MichelucciNeuro-Immunology Group, Department of Cancer Research, Luxembourg Institute of Health, 6A, Rue Nicolas-Ernest Barblé, L-1210 Luxembourg, Luxembourg. Electronic address: alessandro.michelucci@lih.lu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol for quantifying microglial cell morphology at the single-cell level in mice. We provide comprehensive details, starting from optimal mouse brain dissection to computational analyses of up to 350 microglial cells per brain slice. Analyzing the morphology of microglial cells is essential for understanding their functional and activation states in different conditions, including during disease development and progression, as well as for assessing the effect of therapeutic interventions. For complete details on the use and execution of this protocol, please refer to Lind-Holm Mogensen et al.

Indexed as

Fluorescent Antibody TechniqueMicrogliaSingle-Cell AnalysisAnimalsBrainMiceStaining and Labelingbioinformaticscell biologyimmunologyneuroscience

Identifiers

PMID39636729
PMCPMC11653134

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.