Evidence mapPaperPMID 39639377Full record

ArticleEuropean journal of medical research2024

Pulsed radiofrequency alleviates neuropathic pain by upregulating MG53 to inhibit microglial activation.

Yuanxin Huang, Yuanyue Huang, Xianglong Lv, Zilong Yu, Yue Qin, Xingyue Yang, Songsong An, Chunxin Wo, Lin Wang

Abstract read
In one paragraph

Article in European journal of medical research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yuanxin Huang *Pain Department, Affiliated Hospital of Guizhou Medical University, No. 28, Guiyi Street, Liu Guang Men, Guiyang, 550004, Guizhou, China.
Yuanyue Huang *Clinical Medicine School, Guizhou Medical University, Guiyang, 550004, Guizhou, China.
Xianglong LvClinical Medicine School, Guizhou Medical University, Guiyang, 550004, Guizhou, China.
Zilong YuPain Department, Affiliated Hospital of Guizhou Medical University, No. 28, Guiyi Street, Liu Guang Men, Guiyang, 550004, Guizhou, China.
Yue QinPain Department, Affiliated Hospital of Guizhou Medical University, No. 28, Guiyi Street, Liu Guang Men, Guiyang, 550004, Guizhou, China.
Xingyue YangClinical Medicine School, Guizhou Medical University, Guiyang, 550004, Guizhou, China.
Songsong AnClinical Medicine School, Guizhou Medical University, Guiyang, 550004, Guizhou, China.
Chunxin WoPain Department, Affiliated Hospital of Guizhou Medical University, No. 28, Guiyi Street, Liu Guang Men, Guiyang, 550004, Guizhou, China. wochunxin@163.com.
Lin WangPain Department, Affiliated Hospital of Guizhou Medical University, No. 28, Guiyi Street, Liu Guang Men, Guiyang, 550004, Guizhou, China. wanglin0949@yeah.com.

Funding

Guizhou combination- Basics [2023] Key 044Guizhou Provincial Science and Technology Projects 2k[2024]-211
6 · The paper itself

Abstract

backgroundPatients with neuropathic pain (NP) have significantly lower quality of life. Because the pathophysiology of NP is not fully understood, there is a lack of effective treatment for it in clinic. This study set out to investigate the precise mechanism by which pulsed radiofrequency (PRF) alleviated NP.

methodThe rat models of chronic constriction injury of the sciatic nerve (CCI) were established to simulate the occurrence of NP, following with measuring MWT and TWL to evaluate the pain of the rats. HE staining was utilized to observe the rat spinal cord tissue pathology. The expression of MG53, ATF4 and CHOP was evaluated by qRT-PCR and WB, while the expression of inflammatory factors was measured by ELISA. In addition, immunofluorescence assay was used to detect the expression of MG53 and Iba-1.

resultPRF treatment alleviated NP in CCI rats, as well as upregulating the expression of MG53 and inhibiting microglial activation. After MG53 knockdown, the remission of NP by PRF was significantly weakened, but microglial activation and endoplasmic reticulum stress (ERS) exhibited enhancement. Therefore, PRF inhibited microglial activation by upregulating MG53. After injection of ERS inducer in CCI rats, the inhibition effect of overexpressed MG53 on microglial activation and its alleviation effect on NP were reversed. Consequently, MG53 played a role in suppressing microglial activation by mediating the inhibition of ERS.

conclusionPRF attenuated microglial activation by upregulating MG53 to inhibit ERS, resulting in the alleviation of NP in CCI rats.

Indexed as

MicrogliaNeuralgiaPulsed Radiofrequency TreatmentRats, Sprague-DawleyAnimalsDisease Models, AnimalEndoplasmic Reticulum StressMaleRatsUp-RegulationEndoplasmic reticulum stressMG53Microglial activationNeuropathic painPulsed radiofrequency

Identifiers

PMID39639377
PMCPMC11619262

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.