Evidence map›Paper›PMID 39640584›Full record

ArticleiScience2024

Hydrogel bead-based isothermal detection (BEAD-ID) for assessing the activity of DNA-modifying enzymes.

Kathrine Nygaard Borg, Ayush Shetty, Guangyao Cheng, Shaodi Zhu, Tianle Wang, Wu Yuan, Ho Pui Ho, Birgitta Ruth Knudsen, Cinzia Tesauro, Yi-Ping Ho

Abstract read
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In one paragraph

Article in iScience, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Kathrine Nygaard BorgDepartment of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong SAR 00000, China.
Ayush ShettyDepartment of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong SAR 00000, China.
Guangyao ChengDepartment of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong SAR 00000, China.
Shaodi ZhuDepartment of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong SAR 00000, China.
Tianle WangDepartment of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong SAR 00000, China.
Wu YuanDepartment of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong SAR 00000, China.
Ho Pui HoDepartment of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong SAR 00000, China.
Birgitta Ruth KnudsenDepartment of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.
Cinzia TesauroDepartment of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.
Yi-Ping HoDepartment of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong SAR 00000, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA-modifying enzymes are crucial in biological processes and have significant clinical implications. Traditional quantification methods often overlook enzymatic activity, the true determinants of enzymes' functions. We present hydrogel Bead-based Isothermal Detection (BEAD-ID), utilizing uniform hydrogel bead-based microreactors to evaluate DNA-modifying enzyme activity on-bead. We fabricated homogeneous oligo-conjugated polyacrylamide (oligo-PAA) beads

Indexed as

FluidicsMethodology in biological sciencesNanotechnologySensor

Identifiers

PMID39640584
PMCPMC11617385

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.