ArticleJournal of advanced research2025
Hypo-osmolarity promotes naive pluripotency by reshaping cytoskeleton and increasing chromatin accessibility.
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Article in Journal of advanced research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4citing papers in PubMed
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4 citing papers in PubMed.
- Dental Pulp Regeneration: A Comprehensive Review of Stem Cells, Biomaterials, and Bioactive Cues.Stem cell reviews and reports · 2026Review
- Species-specific roles of SP1 in bovine and human embryonic genome activation and early embryonic development.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Novel feeder-cell-free 3C system promotes the transition from primed to formative pluripotency, self-renewal, and germline differentiation in rabbit embryonic stem cellsFrontiers in cell and developmental biology · 2026Article
- SOCS3 deficiency drives the primed to naive pluripotency transition by sustaining STAT3 activation.Frontiers in genetics · 2026Article
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Authors and funding
19 authors.
Renhong LuState Key Laboratory of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Clinical Center for Heart Disease Research, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Arrhythmia Research Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Department of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Frontiers Center of Nanocatalytic Medicine, Shanghai, 200092, China.
Bowen LinState Key Laboratory of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Clinical Center for Heart Disease Research, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Arrhythmia Research Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Department of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Frontiers Center of Nanocatalytic Medicine, Shanghai, 200092, China.
Zheyi LinState Key Laboratory of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Clinical Center for Heart Disease Research, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Arrhythmia Research Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Department of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Frontiers Center of Nanocatalytic Medicine, Shanghai, 200092, China.
Hui XiongState Key Laboratory of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Clinical Center for Heart Disease Research, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Arrhythmia Research Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Frontiers Center of Nanocatalytic Medicine, Shanghai, 200092, China; Department of Pathology and Pathophysiology, School of Medicine, Tongji University, Shanghai 200092, China; Department of Cell Biology, School of Medicine, Tongji University, Shanghai 200092, China.
Junyang LiuState Key Laboratory of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Clinical Center for Heart Disease Research, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Arrhythmia Research Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Frontiers Center of Nanocatalytic Medicine, Shanghai, 200092, China; Department of Pathology and Pathophysiology, School of Medicine, Tongji University, Shanghai 200092, China; Department of Cell Biology, School of Medicine, Tongji University, Shanghai 200092, China.
Li LiInstitute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai 200031, China.
Zheng GongState Key Laboratory of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Jinzhou Medical University, Jinzhou, Liaoning 121000, China.
Siyu WangState Key Laboratory of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Jinzhou Medical University, Jinzhou, Liaoning 121000, China.
Mingshuai ZhangState Key Laboratory of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Clinical Center for Heart Disease Research, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Arrhythmia Research Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Frontiers Center of Nanocatalytic Medicine, Shanghai, 200092, China; Department of Pathology and Pathophysiology, School of Medicine, Tongji University, Shanghai 200092, China; Department of Cell Biology, School of Medicine, Tongji University, Shanghai 200092, China.
Jie DingState Key Laboratory of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Clinical Center for Heart Disease Research, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Arrhythmia Research Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Department of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Frontiers Center of Nanocatalytic Medicine, Shanghai, 200092, China.
Chengwen HangState Key Laboratory of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Clinical Center for Heart Disease Research, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Arrhythmia Research Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Department of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Frontiers Center of Nanocatalytic Medicine, Shanghai, 200092, China.
Huixin GuoDepartment of Cardiology, The Second Hospital of Shanxi Medical University, Taiyuan 030001, China.
Duanyang XieState Key Laboratory of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Clinical Center for Heart Disease Research, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Arrhythmia Research Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Department of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Frontiers Center of Nanocatalytic Medicine, Shanghai, 200092, China; Department of Pathology and Pathophysiology, School of Medicine, Tongji University, Shanghai 200092, China; Department of Cell Biology, School of Medicine, Tongji University, Shanghai 200092, China.
Yi LiuState Key Laboratory of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Clinical Center for Heart Disease Research, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Arrhythmia Research Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Department of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Frontiers Center of Nanocatalytic Medicine, Shanghai, 200092, China.
Dan ShiState Key Laboratory of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Clinical Center for Heart Disease Research, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Arrhythmia Research Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Department of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Frontiers Center of Nanocatalytic Medicine, Shanghai, 200092, China.
Dandan LiangState Key Laboratory of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Clinical Center for Heart Disease Research, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Arrhythmia Research Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Department of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Frontiers Center of Nanocatalytic Medicine, Shanghai, 200092, China; Department of Pathology and Pathophysiology, School of Medicine, Tongji University, Shanghai 200092, China; Research Units of Origin and Regulation of Heart Rhythm, Chinese Academy of Medical Sciences, Shanghai 200092, China.
Zhen LiuInstitute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai 200031, China.
Jian YangState Key Laboratory of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Clinical Center for Heart Disease Research, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Arrhythmia Research Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Department of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Frontiers Center of Nanocatalytic Medicine, Shanghai, 200092, China; Department of Pathology and Pathophysiology, School of Medicine, Tongji University, Shanghai 200092, China; Department of Cell Biology, School of Medicine, Tongji University, Shanghai 200092, China; Research Units of Origin and Regulation of Heart Rhythm, Chinese Academy of Medical Sciences, Shanghai 200092, China. Electronic address: jy279@tongji.edu.cn.
Yi-Han ChenState Key Laboratory of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Clinical Center for Heart Disease Research, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Arrhythmia Research Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Department of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Shanghai Frontiers Center of Nanocatalytic Medicine, Shanghai, 200092, China; Department of Pathology and Pathophysiology, School of Medicine, Tongji University, Shanghai 200092, China; Department of Cell Biology, School of Medicine, Tongji University, Shanghai 200092, China; Research Units of Origin and Regulation of Heart Rhythm, Chinese Academy of Medical Sciences, Shanghai 200092, China. Electronic address: yihanchen@tongji.edu.cn.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionCell fate determination and transition are of paramount importance in biology and medicine. Naive pluripotency could be achieved by reprogramming differentiated cells. However, the mechanism is less clear. Osmolarity is an essential physical factor that acts on living cells, especially for pluripotent cells, but its significance in cell fate transition remains unexplored.
objectivesTo investigate the role of osmolarity in cell fate transition and its underlying mechanism.
methodsFlow cytometry, quantitative real-time PCR, teratoma and chimeric mice assays were performed to assess reprogramming efficiency and characterize iPSCs. TEM, immunofluorescence staining, western blot, chemical treatment and genetic modification were utilized to evaluate cell morphology, signaling pathways, cytoskeleton and nuclear structure. Multiomic sequencings were applied to unveil the transcriptome, histone markers and chromatin accessibility of EpiSCs in hypo-osmotic condition.
resultsIn hypo-osmotic condition, the reprogramming efficiency of hypo-osmotic EpiSCs increased over 60-fold than that of iso-osmotic cells (1100 vs 18 colonies per 3 × 10
conclusionHypo-osmolarity promotes cell fate transition by remodeling cytoskeleton, nucleoskeleton and genome via PI3K-AKT-SP1 pathway.
Indexed as
Cellular ReprogrammingChromatinCytoskeletonInduced Pluripotent Stem CellsAnimalsCell DifferentiationMiceOsmolar ConcentrationSignal TransductionChromatinChromatin accessibilityCytoskeletonOsmolarityPI3K-AKT-SP1 signalingPluripotent stem cellsReprogramming
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LicenceCC BY-NC-ND
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.