Evidence map›Paper›PMID 39650277›Full record

ArticleSovremennye tekhnologii v meditsine2024

Technology of Combined Identification of Macrophages and Collagen Fibers in Liver Samples.

I A Nikitina, V A Razenkova, E A Fedorova, O V Kirik, D E Korzhevskii

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Article in Sovremennye tekhnologii v meditsine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

I A NikitinaJunior Researcher, Laboratory of Experimental Histology and Confocal Microscopy, Department of General and Special Morphology; The Institute of Experimental Medicine, 12 Akademika Pavlova St., Saint Petersburg, 197376, Russia.
V A RazenkovaJunior Researcher, Laboratory of Functional Morphology of Central and Peripheral Nervous System, Department of General and Special Morphology; The Institute of Experimental Medicine, 12 Akademika Pavlova St., Saint Petersburg, 197376, Russia.
E A FedorovaPhD, Researcher, Laboratory of Functional Morphology of Central and Peripheral Nervous System, Department of General and Special Morphology; The Institute of Experimental Medicine, 12 Akademika Pavlova St., Saint Petersburg, 197376, Russia.
O V KirikPhD, Senior Researcher, Laboratory of Functional Morphology of Central and Peripheral Nervous System, Department of General and Special Morphology; The Institute of Experimental Medicine, 12 Akademika Pavlova St., Saint Petersburg, 197376, Russia.
D E KorzhevskiiMD, DSc, Professor of the Russian Academy of Sciences, Head of the Laboratory of Functional Morphology of Central and Peripheral Nervous System; Head of the Department of General and Special Morphology; The Institute of Experimental Medicine, 12 Akademika Pavlova St., Saint Petersburg, 197376, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The importance of identifying pathological changes in the liver both in fundamental researches and in diagnostic practice dictates the necessity to have a convenient method of assessing functional condition of resident macrophages and connective tissue fibers. Materials and Methods: Liver samples from adult rats (n=6) have been used in the study. The connective tissue was stained with a 2% aqueous solution of aniline blue (Unisource Chemicals Ltd., India). Monoclonal rabbit antibodies to Iba-1 (Clone JM36-62; ET1705-78; HuaBio, China) were used to detect resident liver macrophages, zinc-ethanol-formaldehyde was employed as a fixative. Results: The combined staining method allowed us to detect numerous Iba-1-immunopositive structures corresponding morphologically to Kupffer cells and connective tissue macrophages, background staining was not observed. Staining with aniline blue in the liver samples was selective, uniform, and clear, and allowed for differentiation of the connective tissue in all examined samples. Exclusion of the heat-induced epitope retrieval stage caused no negative effect on identification of macrophages, reduced the probability of non-specific staining of the collagen fibers with aniline blue, and ensured general preservation of tinctorial properties of the liver tissue. Conclusion: The presented protocol of combined histo-immunohistochemical identification of Kupffer cells and connective tissue fibers, applied on the rat liver samples, makes it possible to perform effectively the morphometric analysis and may find its application in pathohistological, clinical, and preclinical investigations.

Indexed as

LiverMacrophagesAniline CompoundsAnimalsCalcium-Binding ProteinsCollagenImmunohistochemistryKupffer CellsMaleMicrofilament ProteinsRatsStaining and LabelingAif1 protein, ratAniline CompoundsCalcium-Binding ProteinsCollagenMicrofilament ProteinsIba-1Kupffer cellslivermacrophages

Identifiers

PMID39650277
PMCPMC11618527

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