Evidence map›Paper›PMID 39652878›Full record

ArticleBiochemistry2025

An Alkyne-Containing Isoprenoid Analogue Based on a Farnesyl Diphosphate Scaffold Is a Biologically Functional Universal Probe for Proteomic Analysis.

Shelby A Auger, Jodi S Pedersen, Sanjay Maity, Andrea M Sprague-Getsy, Ellen L Lorimer, Olivia J Koehn, Steven A Krauklis, Brenna Berns, Katherine M Murphy, Jamal Hussain and 9 more

Abstract read
In one paragraph

Article in Biochemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. ACS chemical biology · 2025
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Shelby A AugerDepartment of Chemistry, University of Minnesota-Twin Cities, Minneapolis, Minnesota 55455, United States.ORCID 0000-0003-2873-2580
Jodi S PedersenDepartment of Chemistry, University of Minnesota-Twin Cities, Minneapolis, Minnesota 55455, United States.
Sanjay MaityDepartment of Chemistry, University of Minnesota-Twin Cities, Minneapolis, Minnesota 55455, United States.
Andrea M Sprague-GetsyDepartment of Chemistry, Syracuse University, Syracuse, New York 13244, United States.
Ellen L LorimerDepartment of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, United States.
Olivia J KoehnDepartment of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, United States.
Steven A KrauklisDivision of Nutritional Sciences, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.
Brenna BernsDepartment of Animal Sciences, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.
Katherine M MurphyDepartment of Animal Sciences, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.
Jamal HussainDepartment of Animal Sciences, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.
Pa ThaoDepartment of Chemistry, University of Minnesota-Twin Cities, Minneapolis, Minnesota 55455, United States.
Kaitlyn BernhagenDepartment of Chemistry, University of Minnesota-Twin Cities, Minneapolis, Minnesota 55455, United States.
Katarzyna JustynaDepartment of Chemistry, University of Minnesota-Twin Cities, Minneapolis, Minnesota 55455, United States.
Anjana P SundaresanDepartment of Chemistry, University of Minnesota-Twin Cities, Minneapolis, Minnesota 55455, United States.
Daniel B McKimDivision of Nutritional Sciences, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.
Carol L WilliamsDepartment of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, United States.
James L HouglandDepartment of Chemistry, Syracuse University, Syracuse, New York 13244, United States.ORCID 0000-0003-0444-1017
Ling LiDepartment of Experimental and Clinical Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, United States.
Mark D DistefanoDepartment of Chemistry, University of Minnesota-Twin Cities, Minneapolis, Minnesota 55455, United States.ORCID 0000-0002-2872-0259

Funding

Training Grant: Functional Proteomics of AgingT32AG029796 · NIA · UNIVERSITY OF MINNESOTA · PI EDGAR A ARRIAGA, Douglas G Mashek · 2008 to 2026
$6.6M
Training the Next Generation of Chemical BiologistsT32GM132029 · NIGMS · UNIVERSITY OF MINNESOTA · PI Erin Elizabeth Carlson, William Charles Krause Pomerantz · 2019 to 2026
$2.7M
Chemical Approaches for Exploring Protein Prenylation in Living CellsR35GM141853 · NIGMS · UNIVERSITY OF MINNESOTA · PI MARK D DISTEFANO · 2021 to 2026
$2.4M
Dysregulation of Protein Prenylation in the Pathogenesis of Alzheimer's DiseaseRF1AG056976 · NIA · UNIVERSITY OF MINNESOTA · PI DISTEFANO, MARK D, LI, LING · 2017 to 2017
$1.9M
Determining the scope of prenylatable protein sequencesR01GM132606 · NIGMS · UNIVERSITY OF GEORGIA · PI KANNAN, NATARAJAN, SCHMIDT, WALTER K · 2019 to 2022
$1.6M
NIA NIH HHS RF1 AG056976NIA NIH HHS T32 AG029796NIGMS NIH HHS R01 GM132606NIGMS NIH HHS R35 GM141853NIGMS NIH HHS T32 GM132029
6 · The paper itself

Abstract

Prenylation consists of the modification of proteins with either farnesyl diphosphate (FPP) or geranylgeranyl diphosphate (GGPP) at a cysteine near the C-terminus of target proteins to generate thioether-linked lipidated proteins. In recent work, metabolic labeling with alkyne-containing isoprenoid analogues including C15AlkOPP has been used to identify prenylated proteins and track their levels in different diseases. Here, a systematic study of the impact of isoprenoid length on proteins labeled with these probes was performed. Chemical synthesis was used to generate two new analogues, C15hAlkOPP and C20AlkOPP, bringing the total number of compounds to eight used in this study. Enzyme kinetics performed

Indexed as

AlkynesPolyisoprenyl PhosphatesProtein PrenylationProteomicsAnimalsHumansMiceMolecular ProbesRAW 264.7 CellsSesquiterpenesTerpenesAlkynesfarnesyl pyrophosphateMolecular ProbesPolyisoprenyl PhosphatesSesquiterpenesTerpenes

Identifiers

PMID39652878
PMCPMC11706708

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.