Evidence map›Paper›PMID 39656591›Full record

ArticleSTAR protocols2024

Protocol for quantifying xenografted human cancer cells in zebrafish larvae using Cellpose.

Karina Köpke, Carla-Sophie Lembke, Nynke Oosterhof, Emma S C Dijkstra, Judith T M L Paridaen

Abstract read
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In one paragraph

Article in STAR protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Karina KöpkeEuropean Research Institute for the Biology of Ageing, University Medical Center Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands. Electronic address: k.kopke@umcg.nl.
Carla-Sophie LembkeEuropean Research Institute for the Biology of Ageing, University Medical Center Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands.
Nynke OosterhofEuropean Research Institute for the Biology of Ageing, University Medical Center Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands.
Emma S C DijkstraEuropean Research Institute for the Biology of Ageing, University Medical Center Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands.
Judith T M L ParidaenEuropean Research Institute for the Biology of Ageing, University Medical Center Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol for quantifying xenografted human glioblastoma stem cells (GSCs) in zebrafish larvae. We first describe steps for orthotopic xenotransplantation of GSCs into the midbrain of zebrafish larvae, immunofluorescent labeling, and confocal imaging. We then detail procedures for GSC quantification using the Cellpose algorithm. This protocol provides a technique for the semi-automatic segmentation and quantification of human cancer cells in xenograft experiments. With this approach, cancer cell survival and proliferation can be determined in an unbiased manner.

Indexed as

LarvaZebrafishAlgorithmsAnimalsGlioblastomaHeterograftsHumansNeoplasm TransplantationNeoplastic Stem CellsTransplantation, HeterologousCancerComputer sciencesModel Organisms

Identifiers

PMID39656591
PMCPMC11681912

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.