Evidence mapPaperPMID 39657752Full record

ArticleClinical and molecular hepatology2025

GOLM1 promotes cholesterol gallstone formation via ABCG5-mediated cholesterol efflux in metabolic dysfunction-associated steatohepatitis livers.

Yi-Tong Li, Wei-Qing Shao, Zhen-Mei Chen, Xiao-Chen Ma, Chen-He Yi, Bao-Rui Tao, Bo Zhang, Yue Ma, Guo Zhang, Rui Zhang and 3 more

Abstract read
In one paragraph

Article in Clinical and molecular hepatology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yi-Tong LiHepatobiliary Surgery, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
Wei-Qing ShaoHepatobiliary Surgery, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
Zhen-Mei ChenHepatobiliary Surgery, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
Xiao-Chen MaHepatobiliary Surgery, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
Chen-He YiHepatobiliary Surgery, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
Bao-Rui TaoHepatobiliary Surgery, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
Bo ZhangHepatobiliary Surgery, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
Yue MaHepatobiliary Surgery, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
Guo ZhangHepatobiliary Surgery, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
Rui ZhangHepatobiliary Surgery, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
Yan GengHepatobiliary Surgery, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
Jing LinHepatobiliary Surgery, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
Jin-Hong ChenHepatobiliary Surgery, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.

Funding

National Natural Science Foundation of China 82070655Young Scientists Fund of the National Natural Science Foundation of China 82000605
6 · The paper itself

Abstract

BACKGROUND/

aimsMetabolic dysfunction-associated steatohepatitis (MASH) is a significant risk factor for gallstone formation, but mechanisms underlying MASH-related gallstone formation remain unclear. Golgi membrane protein 1 (GOLM1) participates in hepatic cholesterol metabolism and is upregulated in MASH. Here, we aimed to explore the role of GOLM1 in MASH-related gallstone formation.

methodsThe UK Biobank cohort was used for etiological analysis. GOLM1 knockout (GOLM1-/-) and wild-type (WT) mice were fed with a high-fat diet (HFD). Livers were excised for histology and immunohistochemistry analysis. Gallbladders were collected to calculate incidence of cholesterol gallstones (CGSs). Biles were collected for biliary lipid analysis. HepG2 cells were used to explore underlying mechanisms. Human liver samples were used for clinical validation.

resultsMASH patients had a greater risk of cholelithiasis. All HFD-fed mice developed MASH, and the incidence of gallstones was 16.7% and 75.0% in GOLM1-/- and WT mice, respectively. GOLM1-/- decreased biliary cholesterol concentration and output. In vivo and in vitro assays confirmed that GOLM1 facilitated cholesterol efflux through upregulating ATP binding cassette transporter subfamily G member 5 (ABCG5). Mechanistically, GOLM1 translocated into nucleus to promote osteopontin (OPN) transcription, thus stimulating ABCG5-mediated cholesterol efflux. Moreover, GOLM1 was upregulated by interleukin-1β (IL-1β) in a dose-dependent manner. Finally, we confirmed that IL-1β, GOLM1, OPN, and ABCG5 were enhanced in livers of MASH patients with CGSs.

conclusionIn MASH livers, upregulation of GOLM1 by IL-1β increases ABCG5-mediated cholesterol efflux in an OPN-dependent manner, promoting CGS formation. GOLM1 has the potential to be a molecular hub interconnecting MASH and CGSs.

Indexed as

ATP Binding Cassette Transporter, Subfamily G, Member 5CholesterolFatty LiverGallstonesMembrane ProteinsAnimalsDiet, High-FatDisease Models, AnimalFemaleHep G2 CellsHumansInterleukin-1betaLipoproteinsLiverMaleMiceABCG5 protein, humanABCG5 protein, mouseATP Binding Cassette Transporter, Subfamily G, Member 5CholesterolInterleukin-1betaLipoproteinsMembrane ProteinsATP binding cassette transporter subfamily G member 5GallstonesGolgi membrane protein 1Metabolic dysfunction-associated steatohepatitisMetabolism

Identifiers

PMID39657752
PMCPMC12016653

What Socratic holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.