Evidence map›Paper›PMID 39661682›Full record

ArticleScience advances2024

High-throughput single-cell sorting by stimulated Raman-activated cell ejection.

Jing Zhang, Haonan Lin, Jiabao Xu, Meng Zhang, Xiaowei Ge, Chi Zhang, Wei E Huang, Ji-Xin Cheng

Abstract read
In one paragraph

Article in Science advances, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Jing ZhangDepartment of Biomedical Engineering, Boston University, Boston, MA 02215, USA.ORCID 0009-0007-1892-5086
Haonan LinDepartment of Biomedical Engineering, Boston University, Boston, MA 02215, USA.ORCID 0000-0003-0437-5902
Jiabao XuDivision of Biomedical Engineering, James Watt School of Engineering, University of Glasgow, Glasgow G12 8LT, UK.ORCID 0000-0002-1285-9408
Meng ZhangPhotonics Center, Boston University, Boston, MA 02215, USA.
Xiaowei GePhotonics Center, Boston University, Boston, MA 02215, USA.ORCID 0000-0001-8938-9304
Chi ZhangDepartment of Chemistry, Purdue University, 560 Oval Dr., West Lafayette, IN 47907, USA.ORCID 0000-0002-7735-5614
Wei E HuangDepartment of Engineering Science, University of Oxford, Oxford OX1 3PJ, UK.ORCID 0000-0003-1302-6528
Ji-Xin ChengDepartment of Biomedical Engineering, Boston University, Boston, MA 02215, USA.ORCID 0000-0002-5607-6683

Funding

Vibrational Spectroscopic Imaging to Unveil Hidden Signatures in Living SystemsR35GM136223 · NIGMS · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI CHENG, JI-XIN · 2020 to 2025
$5.7M
Rapid AST through Metabolic Imaging at Single Cell LevelR01AI141439 · NIAID · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI Ji-Xin Cheng · 2019 to 2026
$4.7M
High-content High-speed Chemical Imaging of Metabolic Reprogramming by Integration of Advanced Instrumentation and Data ScienceR01EB032391 · NIBIB · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI CHENG, JI-XIN · 2022 to 2025
$2.0M
NIAID NIH HHS R01 AI141439NIBIB NIH HHS R01 EB032391NIGMS NIH HHS R35 GM136223
6 · The paper itself

Abstract

Raman-activated cell sorting isolates single cells in a nondestructive and label-free manner, but its throughput is limited by small spontaneous Raman scattering cross section. Coherent Raman scattering integrated with microfluidics enables high-throughput cell analysis, but faces challenges with small cells (<3 μm) and tissue sections. Here, we report stimulated Raman-activated cell ejection (S-RACE) that enables high-throughput single-cell sorting by integrating stimulated Raman imaging, in situ image decomposition, and laser-induced cell ejection. S-RACE allows ejection of live bacteria or fungi guided by their Raman signatures. Furthermore, S-RACE successfully sorted lipid-rich

Indexed as

Single-Cell AnalysisSpectrum Analysis, RamanCell SeparationFlow CytometryHigh-Throughput Screening AssaysMicrofluidicsRhodotorula

Identifiers

PMID39661682
PMCPMC11633747

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.