Evidence map›Paper›PMID 39665045›Full record

ArticleCytotechnology2025

Aging and cell expansion enhance microRNA diversity in small extracellular vesicles produced from human adipose-derived stem cells.

Toshiya Tsubaki, Ryota Chijimatsu, Taiga Takeda, Maki Abe, Takahiro Ochiya, Shinsaku Tsuji, Keita Inoue, Tokio Matsuzaki, Yasuhide Iwanaga, Yasunori Omata and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cytotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Toshiya TsubakiOrthopaedic Surgery, Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-Ku, Tokyo, 113-8655 Japan.ORCID 0000-0003-4576-1877
Ryota ChijimatsuOrthopaedic Surgery, Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-Ku, Tokyo, 113-8655 Japan.ORCID 0000-0002-3125-4818
Taiga TakedaCPC Corporation, 3-4 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-0062 Japan.
Maki AbeDepartment of Molecular and Cellular Medicine, Tokyo Medical University, 6-7-1 Nishi-Shinjuku, Sinjuku-Ku, Tokyo, 160-0023 Japan.
Takahiro OchiyaDepartment of Molecular and Cellular Medicine, Tokyo Medical University, 6-7-1 Nishi-Shinjuku, Sinjuku-Ku, Tokyo, 160-0023 Japan.ORCID 0000-0002-0776-9918
Shinsaku TsujiCPC Corporation, 3-4 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-0062 Japan.
Keita InoueCPC Corporation, 3-4 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-0062 Japan.
Tokio MatsuzakiCPC Corporation, 3-4 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-0062 Japan.ORCID 0000-0003-1188-5003
Yasuhide IwanagaOrthopaedic Surgery, Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-Ku, Tokyo, 113-8655 Japan.ORCID 0000-0001-6419-3735
Yasunori OmataOrthopaedic Surgery, Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-Ku, Tokyo, 113-8655 Japan.ORCID 0000-0002-8912-7483
Sakae TanakaOrthopaedic Surgery, Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-Ku, Tokyo, 113-8655 Japan.ORCID 0000-0001-9210-9414
Taku SaitoOrthopaedic Surgery, Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-Ku, Tokyo, 113-8655 Japan.ORCID 0000-0003-4911-3930

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Adipose-derived stem cells (ASCs) and their small extracellular vesicles (sEVs) hold significant potential for regenerative medicine due to their tissue repair capabilities. The microRNA (miRNA) content in sEVs varies depending on ASC status; however, the effects of aging and cell passage on miRNA profiles remain unclear. In this study, we examined the effects of donor age and cell expansion on ASC characteristics and transcriptome using ASCs obtained from three young and three old donors. Cell expansion significantly impaired stem cell properties, notably reducing proliferation and differentiation capacities. In contrast, donor age had minimal effects on ASCs. RNA sequencing (RNA-seq) revealed differences in gene expression related to stemness, phagocytosis, and metabolic processes influenced by cell expansion. To investigate miRNA variability, we performed small RNA-seq on sEVs collected from ASCs of all six donors. The miRNA profiles were influenced by donor age and cell passage. Interestingly, functional enrichment analysis indicated that advanced donor age and increased cell passage may enhance the production of miRNAs associated with organ development through various pathways. These findings suggest that donor age and cell expansion differentially influence ASC characteristics and sEV miRNA content, highlighting the need for disease-specific conditioning of ASCs to optimize the therapeutic effects of sEVs in clinical applications. Supplementary Information: The online version contains supplementary material available at 10.1007/s10616-024-00675-6.

Indexed as

Adipose-derived stem cellsExtracellular vesiclesmicroRNARegenerative therapy

Identifiers

PMID39665045
PMCPMC11631832

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.