Evidence map›Paper›PMID 39668411›Full record

ArticleEuropean journal of immunology2025

Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC.

Diana Dudziak, Lukas Heger, William W Agace, Joyce Bakker, Tanja D de Gruijl, Regine J Dress, Charles-Antoine Dutertre, Thomas M Fenton, Marieke F Fransen, Florent Ginhoux and 20 more

Abstract read
In one paragraph

Article in European journal of immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

30 authors.

Diana DudziakInstitute of Immunology, Jena University Hospital, Friedrich-Schiller-University, Jena, Germany.
Lukas HegerLaboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Erlangen, Germany.ORCID 0000-0001-5591-2187
William W AgaceLEO Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.
Joyce BakkerInstitute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.
Tanja D de GruijlInstitute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.
Regine J DressInstitute of Systems Immunology, Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Charles-Antoine DutertreInserm U1015, Gustave Roussy, Villejuif, France.
Thomas M FentonSchool of Infection and Immunity, University of Glasgow, Glasgow, UK.
Marieke F FransenInstitute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.
Florent GinhouxSingapore Immunology Network (SIgN), Agency for Science, Technology and Research, Singapore, Singapore.
Oded HeymanDepartment of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel.
Yael HorevDepartment of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel.
Florian HornsteinerDepartment of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria.
Vinitha KandiahInstitute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.
Paz KlesDepartment of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel.
Ruth LubinFaculty of Dental Medicine, The Institute of Biomedical and Oral Research, Hebrew University of Jerusalem, Israel.
Gabriel MizrajiDepartment of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel.
Anastasia ProkopiInstitute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.
Or SaarDepartment of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel.
Sieghart SopperInternal Medicine V, Hematology and Oncology, Medical University of Innsbruck, Innsbruck, Austria.
Patrizia StoitznerDepartment of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria.
Helen StrandtDepartment of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria.
Martina M SykoraInternal Medicine V, Hematology and Oncology, Medical University of Innsbruck, Innsbruck, Austria.
Elisa C ToffoliInstitute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.
Christoph H TrippDepartment of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria.
Kim van PulInstitute for Infection and Immunology, Cancer Immunology, Amsterdam, The Netherlands.
Rieneke van de VenCancer Center Amsterdam, Cancer Immunology, Amsterdam, The Netherlands.
Asaf WilenskyDepartment of Periodontology, Hadassah Medical Center, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel.
Simon YonaFaculty of Dental Medicine, The Institute of Biomedical and Oral Research, Hebrew University of Jerusalem, Israel.
Claudia Zelle-RieserDepartment of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria.

Funding

Austrian Academy of Sciences OAW DOC/26015Austrian Research Promotion Agency FFG 858057Austrian Science Fund (FWF) ESP 138-BAustrian Science Fund (FWF) P-21487-B13Austrian Science Fund (FWF) P-27001-B13Austrian Science Fund (FWF) P-33855-BDanish Research Council Sapere Aude III 1331-00136BIsrael Science Foundation 2369/18Lundbeck Foundation R155-2014-4184Singapore National Research Foundation Senior Investigatorship (NRFI) NRF2016NRF-NRFI001-02Swedish Cancerfonden 18 0598Swedish Medical Research Council 2017-02072
6 · The paper itself

Abstract

This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs, and various nonlymphoid tissues. Within this article, detailed protocols are presented that allow for the generation of single-cell suspensions from human nonlymphoid tissues including lung, skin, gingiva, intestine as well as from tumors and tumor-draining lymph nodes with a subsequent analysis of dendritic cells by flow cytometry. Further, prepared single-cell suspensions can be subjected to other applications including cellular enrichment procedures, RNA sequencing, functional assays, etc. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all co-authors, making it an essential resource for basic and clinical DC immunologists.

Indexed as

Dendritic CellsFlow CytometryAnimalsHumansMiceSingle-Cell Analysisdendritic cellsflow cytometrynonlymphoid tissuestumortumor‐draining lymph node

Identifiers

PMID39668411
PMCPMC11739683

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.